Objective:To study the effects of total saponins of Thladiantha dubia root (TSTDR) on spleen index and TNF-α, IL-1β, IL-6 expression in spleen of adjuvant arthritis (AA) rats.Methods:60 male SD rats were randomly divided into the following 6 groups with 10 rats in each group:normal control group, model group, TSTDR low dose (40 mg/kg/d) group, TSTDR middle dose (80 mg/kg/d) group, TSTDR high dose (160mg/kg/d) group and tripterygium glycoside group (12mg/kg/d).TheAArats’model was established by injecting Freund’s complete adjuvant into the plantar subcutaneous layer of right rear foot of rats, and then the rats in each drug group were lavaged with corresponding drugs for 21 days.The spleen index of rats in each group was calculated and immunohistochemical method was used to detect the TNF-α, IL-1β, IL-6 expression in spleen. Results:ThespleenindexofratsinTSTDRmiddleandhighdosegroupwereobviouslylowerthanthatofratsinmodelgroup(P<0.01, P<0.05).TheTNF-α, IL-1β, IL-6 expression in spleen of rats in eachTSTDR group were all significantly lower than model group (P<0.01, P<0.05);Moreover,TheTNF-α, IL-1β, IL-6 expression in spleen of rats inTSTDR middle and high dose group were significantly lower than TSTDR low dose group (P<0.01, P<0.05).Conclusions:Inhibition of TNF-α, IL-1βandIL-6expressioninspleenmaybeoneofthemechanismsofTSTDRfortreatingAA.%目的：观察赤雹根总皂苷(TSTDR)对佐剂性关节炎(AA)大鼠脾脏指数和脾脏肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)表达的影响。方法：雄性SD大鼠60只随机分为正常对照组，模型组，TSTDR低(40mg/kg/d)、中(80mg/kg/d)、高(160mg/kg/d)剂量组和雷公藤多苷组(12mg/kg/d)。大鼠右后足跖部皮下注射弗氏完全佐剂0.1ml建立AA大鼠模型，成模后各用药组大鼠分别灌胃给予相应药物21d。计算大鼠的脾脏指数，免疫组织化学染色法检测各组大鼠脾脏TNF-α、IL-1β、IL-6的表达。结果：TSTDR中、高剂量组大鼠的脾脏指数显著低于模型组(P＜0.01，P＜0.05)。TSTDR各剂量组大鼠脾脏TNF-α、IL-1β、IL-6的表达水平明显低于模型组(P＜0.01，P＜0.05)；并且，TSTDR中剂量组、TSTDR高剂量组大鼠脾脏TNF-α、IL-1β、IL-6的表达水平明显低于TSTDR低剂量组(P＜0.01，P＜0.05)。结论：抑制脾脏TNF-α、IL-1β、IL-6的表达可能是TSTDR治疗AA的机制之一。
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