目的：观察不同煎煮时间大黄水提物对幼年雌性大鼠子宫、卵巢和体重指数的影响。方法：4周龄雌性SD大鼠70只，随机分为正常对照组，大黄水煎10min剂0.5g/kg、1.0g/kg、2.0g/kg组，大黄水煎30min剂0.5g/kg、1.0g/kg、2.0g/kg组，各组大鼠均灌胃给药30天。分析各组大鼠的子宫、卵巢指数以及体重指数。结果：大黄水煎10min各剂量组，大黄水煎30min 2.0g/kg组大鼠子宫、卵巢、体重指数明显低于正常对照组(P＜0.05)；相同煎煮时间不同剂量比较：水煎30min 2.0g/kg组大鼠子宫、卵巢、体重指数低于0.5g/kg组(P＜0.05)，水煎10min 2.0g/kg、1.0g/kg组大鼠子宫、卵巢、体重指数低于0.5g/kg组(P＜0.05)；不同煎煮时间相同剂量比较：水煎10min各剂量组大鼠子宫、卵巢、体重指数低于30min各剂量组(P＜0.05)。结论：不同煎煮时间大黄水提取物可对幼年雌性大鼠体重增长、子宫和卵巢指数造成一定影响，影响程度与大黄的煎煮时间和剂量有关。%Objective:To observe the inlfuence of Rhubarb water extract in different decocting time on uterus index, ovary index and body mass index. Methods:70 4-week old female SD rats were randomly divided into normal control group, Rhubarb 0.5g/kg, 1.0 g/kg and 2.0 g/kg group respectively decocting 10min and 30min. All the rats were lavaged with corresponding drugs for 30 days and then detected the uterus index, ovary index and body mass index of rats in each group. Results:The uterus index, ovary index andbody mass index of rats in each dose group of Rhubarb decoction 10min, 2.0g/kg group of Rhubarb decoction 30min were obviously lower than that of rats in normal control group (P<0.05).Different dose Rhubarb of same decocting time:decoction 30min, the uterus index, ovary index and body mass index of rats in 2.0g/kg group were obviously lower than that of rats in 0.5g/kg group (P<0.05);decoction 10min, the uterus index, ovary index and body mass index of rats in 2.0g/kg and 1.0g/kg group were obviously lower than that of rats in 0.5g/kg group (P<0.05). Different decocting time of same dose Rhubarb:The uterus index, ovary index and body mass index of rats in each dose group of decoction 10min were obviously lower than that of rats in each dose group of decoction 30min (P<0.05). Conclusions:Rhubarb water extract in different decocting time can cause certain inlfuences on uterus index, ovary index and weight gain, and the impacts are related to decocting time and dose.
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