目的：研究大肠癌谷胱甘肽-巯基-转移酶-π(GST-π)和P-糖蛋白(P-gp)的表达情况及二者与大肠癌临床病理特征的关系。方法：应用免疫组织化学S-P法检测86例大肠癌组织和52例远端正常组织(距癌灶＞10cm)GST-π和P-gp的表达情况，并分析二者与大肠癌临床病理特征的关系。结果：大肠癌组织GST-π和P-gp的阳性表达率均明显高于远端正常组织(P＜0.01，P＜0.05)。大肠癌组织P-gp表达与淋巴结转移有关，有淋巴结转移者P-gp阳性表达率明显高于无淋巴转移者(P＜0.05)。大肠癌GST-π和P-gp的表达呈正相关(P＜0.01)。结论：GST-π、P-gp在大肠癌中的高表达可能与大肠癌原发性多药耐药有关，临床联合检测GST-π和P-gp可为制定合理的个体化化疗方案提供理论依据。%Objective:To study the expression of glutathione-s-transferase-π(GST-π) and P-glycoprotein (P-gp) in human colorectal cancer and their relationships with clinical pathological characteristics of colorectal cancer. Methods:S-P immunohistochemical staining was used to detect the expression of GST-πand P-gp in 86 cases colorectal cancer and 52 cases distal normal tissue (>10cm from the cancer). And the correlations between GST-π, P-gp expression and the clinical pathological characteristics of colorectal cancer were analyzed.Results:The positive expression rate of GST-πand P-gp in colorectal cancer were all signiifcantly higher than that in distal normal tissues (P<0.01, P<0.05). The expression of P-gp in colorectal cancer was related with lymph node metastasis:the positive expression rate of P-gp of patients with lymph node metastasis was signiifcantly higher than that no lymph node metastasis (P<0.05). There had positive correlation between GST-πand P-gp expression in colorectal cancer (P<0.01). Conclusions: High expression of GST-πand P-gp in colorectal cancer may be associated with primary multidrug resistance of the cancer. Joint detection of GST-πand P-gp in clinic can provide theoretical basis for reasonable individual chemotherapy scheme.
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