飞行时间二次离子质谱(TOF-SIMS)成为材料表面化学分析越来越重要的手段,随着分析仪器性能的不断提高,尤其是团簇离子源的发明和使用,使得TOF-SIMS在生物材料和生命科学研究中能够更接近常规性地被使用.它可以用来鉴定表面的生物分子,并且描述生物分子在单细胞表面和内部及组织切片上的二维分布.TOF-SIMS的主要测试功能包括表面质谱,化学成像及深度剖析3种.本综述(分上、下两篇)围绕这3项仪器功能,简单综述近20年内TOF-SIMS在生物材料和生命科学中的应用.本篇主要讨论应用质谱功能表征生物医学有机高分子材料表面化学特性及在表面的生物分子,包括氨基酸、多酞、蛋白质、核苷酸、DNA、磷脂膜及多糖.重点举例介绍的科学问题包括蛋白质吸附、生物材料表面化学改性以及生物降解高分子药物释放机理.%Time-of-flight secondary ion mass spectrometry(TOF-SIMS)is becoming an important technique for chemical surface analysis since its instrumental performance has been improved. The discovery and application of the cluster ion beams in the past few years promote TOF-SIMS to be used almost as a routine analytical tool for identifying bio-molecules at surface and mapping their 2D distributions at the surface or in the interior of cells and on the cross-section of tissues. The major analytical functions of TOF-SIMS include surface mass spectrometry, chemical mapping and depth profiling. This review (divided into part I and part II) gives brief introduction about how these three analytical functions were applied to solve the problems in biomaterials research and life science during the past two decades. This paper as the first part of the complete review is focused on describing the applications of TOF-SIMS as a surface mass spectrometry to characterize biomedical organic polymermaterials surface and common biomolecules including amino acid, peptide, protein, nucleo-tide, DNA, lipid and sugar at surface. In addition, some examples are given to demonstrate the applications of TOF-SIMS in studying protein adsorption mechanism, chemical surface modification of biomaterials and investigating drug release mechanism of biodegradable polymer.
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