HPLC-MS/MS method was established for the determination of hydrocodone in human plasma. Diazepam was used as internal standard, methanol-0.5% formic acid water was used as the mobile phase for gradient elution, Agilent-C18 column (2.1×50 mm×3. 5 μm) was used as analytical column. Electrospray ionization(ESI) source was applied, and multiple reaction monitoring(MRM) mode was operated in the positive mode with the monitor ions at m/z 300. 2→199. 1 for hydrocodone and m/z 285. 0→193. 0 for the internal standard, respectively. The linear calibration curve is obtained over the concentration range of 0. 5-50 μg/L. The limit of quantitation is 0. 5 μg/L(n = 6). The inter and intra-day pre-cision(RSD) is less than 15%. The average recoveries are above 75%. The method is proved to be specific, convenient, sensitive and suitable for pharmacokinetics of hydrocodone.%建立HPLC-MS/MS方法定量测定人血浆中氢可酮浓度.以地西泮为内标,采用甲醇-0.05％甲酸水溶液为流动相进行梯度洗脱,以Agilent-C18(2.1×50 mm×3.5 μm)色谱柱为分析柱,通过电喷雾离子源( ESI),以正离子多反应监测(MRM)方式进行检测.用于定量分析的离子对分别为m/z 300.2→199.1(氢可酮)和m/z 285.0→193.0(地西泮,内标).氢可酮线性范围为0.5～50μg/l,定量下限为0.5 μg/L(n=6),日内、日间精密度的RSD均小于15％,平均回收率大于75％.本方法专属性强、样品处理简便、灵敏度高,适用于氢可酮临床药动学研究.
展开▼
