Suppression of intracranial glioma tumorigenesis with vascular endothelial growth factor antisense oligonucleotide in rats

著录项

• 来源
  《军事医学研究：英文版》 |2003年第4期|242-245|共4页
• 作者

  李维方; 张光霁; 朱诚; 金由辛; 卢亦成;

  展开▼
• 作者单位

  Department of Neurosurgery;

  Changzheng Hospital;

  Second Military Medical University;

  Shanghai 200003;

  China;

  State Key Laboratory of Molecular Biology;

  Institute of Biochemistry and Cell Biology;

  Shanghai Institute of Life Sciences;

  Chinese Academy of Sceinces;

  Shanghai 200032;

  China;

  Department of Neurosurgery;

  Changzheng Hospital;

  Second Military Medical University;

  Shanghai 200003;

  Chinabjective: To observe the inhibition of intracranial glioma tumorigenesis by vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide (ODN) in rats. Methods: Totally 20 μ1 Hank's liquid containing 1×106 C6 glioma cells was seeded into rat right caudate putaraen in high-flow microinfusion with stereotactic technique. VEGF antisense ODN was simultaneously used with glioma cell. Each rat of the treated group Ⅰand the treated group Ⅱwas treated with 1 000 μmol/L VEGF antisense ODN. Each rat of the treated group Ⅲand the treated group Ⅳwas treated with 2 000 μmol/L VEGF antisense ODN. The experimental periods of the treated group Ⅰ;

  the treated group Ⅲand the control group Ⅰwere 2 weeks;

  those of the treated group Ⅱ;

  the treated group Ⅳand the control group Ⅱwere 3 weeks. Before sacrifice;

  MRI was performed on each rat. Tumor magnitude and pathologic examination were detected after samples were dissected. Results: The survival state of all treated rats was better;

  and that of the control rats was in severe danger. The tumor volumes of the treated group Ⅰand the treated group Ⅱwere remarkably lessened. Tumor tissue could not be found macroscopically in the brain samples of the treated group Ⅲand the treated group Ⅳ;

  but tumor nest could be found with microscopy. Tumors of the treated group I and the treated group Ⅱhad weak expressions of VEGF mRNA and VEGF;

  while normal brains and the samples of the treated group Ⅲand the treated group Ⅳhad negative expressions;

  but tumors of the control groups had strong expressions. Conclusion: VEGF antisense ODN used early in situ can suppress angiogenesis and growth of rat intracranial glioma to retard tumorigenesis.;

  展开▼
• 原文格式 PDF
• 正文语种 chi
• 中图分类 神经组织瘤;
• 关键词

  颅内神经胶质瘤; 肿瘤发生; 血管内皮生长因子; 反义寡核苷酸;

  机译：反义寡核苷酸;抗血管生成;神经胶质瘤;抑制;VEGF;大鼠;