A simple and accurate high-performance liquid chromatography (HPLC) method to generate the fingerprints of crude Pu-erh tea (CPT) and ripened Pu-erh tea (RPT) is described. A suitable chromatographic system was established using a linear gradient elution with acetonitrile and water containing 0.6% formic acid as the mobile phase and a detection wavelength of 300 nm. HPLC analysis identified 24 common peaks among RPT and 21 common peaks among CPT. This study revealed that crude Pu-erh tea and ripened Pu-erh tea contained some similar major constituents, but with distinctive peaks, which may be caused by the difference in the fermentation process. This HPLC fingerprint method could be used to evaluate and authenticate crude Pu-erh tea and ripened Pu-erh tea.%本文首次建立了一种简单而准确的高效液相色谱指纹图谱方法,对不同产地的普洱生茶和普洱熟茶进行了系统评价.采用线性梯度洗脱,乙腈和0.6%甲酸水为流动相,柱温30℃,检测波长为300 nm.该高效液相色谱指纹图谱分析表明,不同产地的普洱熟茶和普洱生茶分别有24个共有峰和21个共有峰.研究结果显示普洱生茶和普洱熟茶的部分共有峰相同,但是两者不同的发酵工艺又产生了各自特征性的指纹峰.因此,此高效液相色谱指纹图谱可以用于普洱熟茶和普洱生茶的评价和真伪鉴别.
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