目的：确定生殖道衣原体感染（GCI）所致氧化应激中的生物标志物和性激素在输卵管阻塞中的作用。方法前瞻性病例对照研究，纳入150例年龄配对的女性，分为三组：衣原体阳性的不孕女性（n =50）；衣原体阳性的可孕女性（n =50）以及作为对照的衣原体阴性的可孕女性（n =50）。通过收集高位阴道拭子（HVS）和宫颈拭子（ECS）检测沙眼衣原体抗原；收集血清检测女性性激素[卵泡刺激素（ FSH）、黄体生成素（LH）、雌二醇（E2）、孕酮（P4）、催乳素（PRL）]以及检测氧化应激的生物标记物[总抗氧化能力（TAC）和8-羟基-2-脱氧鸟苷（8- OHdG）]。结果各组间患者的 FSH、PRL 和 E2水平均无显著差异（ P >0.05）。在患有 GCI 的女性中，衣原体阳性的不孕女性较衣原体阳性的可孕女性 LH 和8- OHdG 水平显著升高（ P <0.05）；不孕的衣原体阳性女性较衣原体阴性的可孕女性，其 LH 和8-OHdG 水平升高而 TAC 水平降低。结论 DNA 氧化损伤和抗氧化能力减弱等机制可能与衣原体引起的输卵管损伤有关。%Objective The purpose of the study was to determine the possible contribution of biomarkers of oxidative stress and female re-productive hormones in genital Chlamydial infection to tubal occlusion. Methods In this prospective case control study,150 age - matched women were grouped into infertile Chlamydia positive women(n = 50),fertile Chlamydia positive women(n = 50)and fertile Chlamydia negative women as controls(n = 50). High vaginal swabs and endocervical swabs were collected for testing Chlamydia trachomatis,Trichomonas vaginalis, Treponema pallidum,Staphylococcus aureus,and Candida albicans. Sera were collected for testing Chlamydia trachomatis antibody,female repro-ductive hormones Follicle Stimulating Hormone(FSH),Luteinizing Hormone(LH),Oestradiol(E2),Progesterone(P4),Prolactin(PRL)〗, and testing biomarkers of oxidative stress[Total Antioxidant Capacity(TAC)and 8 - hydroxyl - 2 - deoxyguanosine(8 - OHdG)]by enzyme im-munoassay(EIA). Results The levels of LH and 8 - OHdG were higher in infertile Chlamydia positive women compared to fertile Chlamydia positive women( P < 0. 05). The levels of LH and 8 - OHdG were higher and the levels of TAC were lower in infertile Chlamydia positive women compared to fertile Chlamydia negative women as controls( P < 0. 05). Conclusion Oxidative DNA damage and reduced antioxidant capacity may be involved in the pathology of Chlamydia induced tubal damage.
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