Objective To observe the effects of percutaneous trigeminal nerve electrical stimulation on behavior and the ability of learning and memory of epileptic rats induced by Pilocarpine.Methods Thirty healthy male rats were randomly divided into normal control group, epilepsy model group and trigeminal nerve stimulation group ( TNS group) .Pilocarpine was used to inducestatus epileptics ( SE) in rats.TNS group rats were treated with percutaneous trigeminal nerve stimulation consecutive for 4 weeks.At 2 weeks after SE model established, video monitor were employed to record the frequency of spontaneous recurrence seizures ( SRS) , the intensity and duration of seizures. At 4 weeks after SE model established, rats in each group were taken Morris water maze test.The incubation period of rats finding underwater platform in the acquired training was recorded.The percentage of time and distance of rats searching target quadrant and the number of times through the platform were also recorded.Results Rats in control group didn''t show abnormal behavior throughout this experiment.During the process of animal model making, a rat died in epilepsy model group while 2 in TNS group.During-EEG monitoring, 2 rats in epilepsy model group died. Compared with epilepsy model group, the frequency of spontaneous recurrence seizure and the intensity of seizure significantly decreased, the average time of seizure significantly shortened in TNS group ( P<0.05 -0.01 ) in 2 weeks.In acquired training experiment, with the number of training increased, the average escape latent of experimental animals gradually reduced, the escape latent of rats in epilepsy model group were significantly prolonged compared with control group rats at each time-point ( all P<0.01 ) , while the average escape latency was significantly reduced in TNS group rats than epilepsy model group (P<0.05-0.01).Compared to control group, the percentage of time search and the percentage of navigation distance in the target quadrant and the number of cross platform were significantly decreased in epilepsy model group and TNS group rats (all P<0.01).Compared to epilepsy model group, the percentage of time search and the percentage of navigation distance in the target quadrant within 60s were significantly higher and more cross platform numbers in TNS group ( P<0.05-0.01) .Conclusion In experimental epilepsy, percutaneous trigeminal nerve stimulation can reduce spontaneous recurrence seizures of the temporal lobe epilepsy rat and protect the ability of learning and memory.%目的 探讨经皮三叉神经电刺激对匹罗卡品诱导的癫痫大鼠的行为及学习记忆能力的影响.方法 健康雄性大鼠30只,随机平均分成正常对照组、癫痫模型组和经皮三叉神经电刺激组( TNS组). 采用匹罗卡品诱导癫痫持续状态制作大鼠模型. TNS组大鼠给予经皮三叉神经电刺激处理4周. 模型建立2周后,视频脑电监测大鼠自发性发作的频率、发作强度以及持续时间. 模型建立4周后,各组大鼠进行Morris水迷宫试验,记录大鼠在获得性训练中寻找水下平台的逃避潜伏期,以及在空间探索试验中搜索目标象限的时间百分比、航行距离百分比和穿越平台的次数. 结果 正常对照组大鼠在实验全程均未出现异常行为. 动物模型制作过程中,癫痫模型组有1只大鼠死亡,TNS组有2只大鼠死亡. 视频脑电监测期间,癫痫模型组有2只大鼠死亡. 与癫痫模型组比较,TNS组大鼠2周内自发性发作频率明显降低,发作持续时间明显缩短,发作级别评分明显降低( P<0.05~0.01). 在获得性训练中,随着训练天数的增加,各组大鼠平均逃避潜伏期逐渐缩短;与正常对照组比较,癫痫模型组大鼠各时间点平均逃避潜伏期均明显延长(均P<0.01 );与癫痫模型组比较, TNS组大鼠各时间点平均逃避潜伏期均明显缩短( P<0.05~0.01). 与正常对照组比较,癫痫模型组和TNS组大鼠在目标象限搜索的时间百分比及航行距离百分比明显降低,穿越平台的次数明显减少(均P<0.01);与癫痫模型组比较,TNS组大鼠在目标象限搜索的时间百分比及航行距离百分比显著增高,穿越平台的次数明显增多( P<0.05~0.01). 结论 经皮三叉神经电刺激可减少匹罗卡品诱导的癫痫大鼠的自发性发作,保护其学习记忆能力.
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