目的 探讨细胞色素p4502E1基因(CYP2EI)和谷胱甘肽转硫酶MI(GSTM1)基因多态性与深圳地区非小细胞肺癌遗传易感性的相关性.方法 收集2014年2月~2016年10月在深圳各医院就诊并确诊为非小细胞肺癌患者和同期住院的肺良性疾病患者各71例,采用PCR-RFLP和PCR法分别检测CYP2E1基因的RsaⅠ/PstⅠ和GSTM1基因多态性,并分析基因多态性与非小细胞肺癌遗传易感性之间的相关性.结果 非小细胞肺癌组和肺良性疾病组患者CYP2E1基因Rsa I/Pst I多态性的三种基因型检出频率差异无统计学意义(χ2=0.891~1.205,P>0.05);非小细胞肺癌组GSTM1(–)基因型频率为61.79%,显著高于肺良性疾病组的36.62%,两者频率的差异有统计学意义(χ2=5.019,P0.05) non-small cell lung cancer and lung benign disease group patients in GSTM1 gene polymorphism,CYP2E1 gene Rsa I/Pst I polymorphisms of the three genotypes detection.;In non-small cell lung cancer group,GSTM1(–) genotype frequency was 61.79%,significantly higher than 36.62% of lung benign disease group, the difference was statistically significant in genetype frequency between two group (χ2=5.019,P<0.05);The risk of non-small cell lung cancer is significantly higher in individual carrying GSTM1(–) genotypes than the GSTM1 (+) genotype individuals OR 2.095 (95% CI=1.104~3.173,P=0.032);with cl/c2 and c2/c2 gene type of nonsmoking individuals,carrying the cl/cl genotypes of smokers significantly increased risk of non-small cell lung cancer (OR= 3.415,95% CI=1.092~11.214,P=0.028); with cl/cl and GSTM1 (–) genotype individuals had a significantly higher risk of non-small cell lung cancer than individuals with GSTM1 (+) and cl/c2 and c2 / c2 genotype ((OR=3.518, 95% CI=3.518~l2.812,P=0.045).In the crowd,smoking is not allowed to carry GSTM1 (–) and cl/cl genotype had a significantly higher risk of non-small cell lung cancer with GSTM1 (+) and cl/c2 and c2 / c2 gene type of people (OR=2.917,95% CI=1.004~8.316,P=0.043),and with GSTM1 (–) and cl/c2 and c2 / c2 gene type of crowd is higher than the same risk of non-small cell lung cancer with GSTM1 (+) and cl/c2 and c2 / c2 gene type of people (OR=14.062,95% CI=1.362~147.256,P=0.029).Conclusion GSTM1 genotypes (–) is regarded as one of the risk factors for non small cell lung cancer were diagnosed in shenzhen area ; At the same time carrying CYP2E1 cl/cl and GSTM1 (–) genotypes can increase risk of non-small cell lung cancer in the smoking and nonsmoking population.
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