Objective To observe the inhibitory effect of probiotics mixture on patients with ulcerative colitis NF-kappa B signaling pathway and clinical efficacy.To study on the inhibitory effect of probiotics mixture on patients with ulcerative colitis NF-κB signaling pathway,the clinical efficacy was also observed.Methods 80 patients with ulcerative colitis were randomly divided into observation group and control group with 40 cases in each group.40 cases,The control group were routinely treated with take treatment of ulcerative colitis (mesalazine + prednisone),and the observation group was treated with a mixture of probiotics (glutamine + triple viable + deep sea fish oil).After 2 courses of treatment,the levels of inflammatory cytokines IL-1,IL-6 and TNF-α as well as nuclear factor kappa B (NF-κB) subunit p65,p50 expression and inhibition of Kaba protein (IkB-α) were compared between the two groupsA comparison of,The curative effects and adverse reactions were observed.Results The total effective rate of the observation group was significantly higher than that of the control group (P ＜ 0.05),and the expression of he IL-1,IL-6 and TNF-of the two groups were significantly lower than those before treatment (P ＜ 0.05).IL-1 and IL-6 in the observation group after treatment were significantly lower than those in the control group (P ＜ 0.05).In the observation group,the levels of NF-κB/p65 and NF-κB/p50 were lower than that before treatment and the control after treatment(P ＜ 0.05),and IkB-α was significantly higher than that before treatment and the control after treatment (P ＜ 0.05).There was no significant difference in the incidence of adverse reactions between the two groups (P ＞0.05).Conclusion A mixture of probiotics can downregulate the NF-kappa B/p65,NF-kappa B/p50 expressionelevate IkB-alpha level,and reduce the expression of inflammatory cytokines in patients with ulcerative colitis,which benefits the clinical treatment and is safe and reliable.%目的 观察研究益生菌混合物对溃疡性结肠炎患者NF-κB信号通路的抑制作用及临床疗效.方法 将本院80例溃疡性结肠炎患者随机分为观察组与对照组,每组各40例,对照组采取溃疡性结肠炎常规治疗(美拉沙嗪+醋酸泼尼松),观察组加用益生菌混合物(谷氨酰胺+三联活菌+深海鱼油)干预,治疗2疗程后比较两组细胞炎性因子IL-1β、IL-6、TNF-α水平差异,另比较核因子-κB (NF-κB)亚单位p65、p50表达及抑制性卡巴蛋白(IkB-α)水平,同时观察疗效及不良反应.结果 观察组总有效率显著高于对照组(P＜0.05).两组IL-1β、IL-6、TNF-α与治疗前比较显著较低(P＜0.05),观察组治疗后IL-1β、IL-6与对照组比较显著较低(P＜0.05);观察组治疗后NF-κB/p65、NF-κB/p50与治疗前及对照组治疗后比较显著降低(P＜0.05),IkB-α与本组治疗前及对照组治疗后比较显著增高(P＜0.05).两组不良反应发生率比较差异无统计学意义(P＞0.05).结论 益生菌混合物辅助治疗可有效下调溃疡性结肠炎患者NF-κB/p65、NF-κB/p50表达,增高IkB-α水平,可降低溃疡性结肠炎患者细胞炎性因子表达水平,使临床治疗获益更多,且安全可靠.
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