Optimization of Adsorption Conditions of Papain on Cross-Linked Phage-Displayed Heptapeptide Matrix Using Response Surface Methodology

杨慧琴; 陈天翔; 刘琳; 陈德强; 李树白; 权静; 朱利民; 聂华丽

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Optimization of Adsorption Conditions of Papain on Cross-Linked Phage-Displayed Heptapeptide Matrix Using Response Surface Methodology

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A new cross-linked heptapeptide matrix(CHM)was prepared and the adsorption performance for papain was investigated.Firstly,a phage-displayed heptapeptide(Ile-Gln-SerPro-His-Phe-Phe)with high affinity to papain was found.Secondly,the heptapeptide was cross-linked to a matrix for papain adsorption.Finally,the adsorption conditions were optimized by response surface methodology.The results indicated that the optimum adsorptionconditionsweredeterminedasinitialpH 6.9,temperature 40℃,and the adsorption capacity of CHM for papain was found to be 55.52 mg/g.Moreover,the papain was purified 78-fold in a single step determined by affinity precipitation.More than87%of the adsorbed papain was desorbed using the eluent solventtriethylamine at pH 7.4.The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.

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《东华大学学报：英文版》 |2017年第1期|111-117|共7页
作者
杨慧琴; 陈天翔; 刘琳; 陈德强; 李树白; 权静; 朱利民; 聂华丽;
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作者单位

Key Laboratory of Textile Science & Technology;

Ministry of Education;

Donghua University;

Shanghai 201620;

China;

College of Chemistry;

Chemical Engineering and Biotechnology;

Donghua University;

Shanghai 201620;

China;

Changzhou Institute of Engineering Technology;

Changzhou 213164;

China;

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原文格式 PDF
正文语种 chi
中图分类 Q184.1;
关键词
heptapeptide; papain; response surface methodology; adsorption; affinity precipitation;

Optimization of Adsorption Conditions of Papain on Cross-Linked Phage-Displayed Heptapeptide Matrix Using Response Surface Methodology

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