The development of a 16-channel electrophysiological signal detecting system based on the microelectrode array is described. The detecting system consists of hardware and software components, and the hardware can be divided into the following three modules: Microelectrode array interface module, which is used for connection of the micro-electrode array and the detecting system; Multi-channel signal amplification module, which is used for signal amplification; Data acquisition module, which is used for high speed data acquisition. The software design uses multi-threaded and multi-caching technology to ensure the data's real-time observation and analysis. The main parameters of the detecting system are tested, and the laboratory-made microelectrode array is used for the detection of acute hippocampus slices. The system's input noise Vrms, is less than 2 μV, total gain of the amplifier is 1000, frequency band is 10-3000 Hz. The neural electrophysiological signals of brain slice are successfully detected. With the proper design of hardware and software system, the voltage revolution of system can attain 0.6 μV which can meet the demand of neural electrophysiological signal detection.%该文设计了基于微电极阵列的16通道神经电生理信号检测系统.检测系统由硬件和软件两部分组成,其中硬件部分可分为以下3个模块:微电极阵列接口模块,用于实现微电极阵列和检测系统的可靠连接;多通道信号放大模块,用于对微弱电生理信号进行提取并放大至合适的幅度；数据采集模块,对放大后的电生理信号进行高速数据采集并通过USB2.0接口和计算机相连.软件部分采用多线程、多缓存等技术保证对信号的实时观测和分析.对检测系统的主要参数进行了测试,并结合实验室自制神经微电极阵列对SD大鼠海马区脑切片进行神经电生理信号的检测.系统的输入噪声Vrms＜2 μV,放大倍数为1000倍,频率带宽范围为10～3000 Hz,并且能够检测到放电幅度为20μV左右的神经电生理信号.该文针对微电极阵列神经电生理信号检测中的技术难点,从硬件和软件设计上保证微弱信号的提取,检测系统的分辨率可达0.6 μV,各项参数能够满足神经电生理信号的检测需要.
展开▼
