研究不同温度(10、15、20、25、30℃,光照强度80μmol·m–2·s–1,盐度30)、光照强度(20、60、100、200、300μmol·m–2·s–1,培养温度20℃,培养液盐度30)和盐度(盐度10、20、30、40和50,培养温度20℃,光照强度80μmol·m–2·s–1)对瓦氏马尾藻(Sargassum vachellianum)生长、光合色素含量及光合放氧活性的影响。结果表明,3种环境因子对瓦氏马尾藻生长、光合色素含量及光合放氧活性影响显著(P<0.05)。其中,瓦氏马尾藻适宜生长条件为：温度15~20℃,最适温度为20℃；光照强度20~60μmol·m–2·s–1；盐度20~40,最适盐度为30。最高特定生长率达5.80%·d–1。温度高于25℃或光照强度大于200μmol·m–2·s–1或盐度小于10或大于50藻体2两周后基本停止生长并出现发白、变软、腐烂现象。温度10~20℃、光照强度20~60μmol·m–2·s–1、盐度20~40时较适宜瓦氏马尾藻光合色素的积累。温度20℃、光照强度100μmol·mg–1·h–1、盐度30时瓦氏马尾藻的光合放氧活性最高,最高值达258.50μmol·m–2·s–1。与低光强相比高光强对瓦氏马尾藻光合放氧活性的抑制作用不明显。研究结果为瓦氏马尾藻的栽培和藻场修复提供了理论依据。%We studied the effects of different temperatures, light intensity and salinity on the specific growth rate, dry weight, photosynthetic pigment content, and photosynthetic oxygen evolution in Sargassum vachellianum. Temperature, light intensity and salinity all had a significant effect on growth, photosynthetic pigment content, and photosynthetic oxygen evolution. The most suitable temperatures, light intensity and salinity for S. vachellianum were 15–20℃, 20–60 μmol·m–2·s–1and salinity 20–40, respectively. The optimal growth temperature and salinity was 20℃and 30, the maximum specific growth rate reaching 5.80%· d–1. When the temperature was above 25℃, or the light intensity was higher than 200 μmol·m–2·s–1, or the salinity was less than 10 or higher than 50, the blades would stop growing, become pale and soft, and then rot 2 weeks later. Sargassum vachellianum could accu-mulate more photosynthetic pigment when the temperature was between 10–20℃or the l i g ht intensity was at 20–60μmol·m–2·s–1 or the salinity was at 20–40. When S. vachellianum was cultured at 20℃, a light intensity of 100μmol·m–2·s–1, and salinity 30, photosynthetic oxygen evolution from the blades was highest, about 258.50μmol·mg–1·h–1. Compared with the lower light intensity, the higher light intensity inhibited photosynthetic oxygen evolution. The results provide a theoretical basis for cultivation and restoration of S. vachellianum.
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