以人工繁育大麻哈鱼(Oncorhynchus keta)发眼后胚胎为研究对象,设置4个茜素红染料质量浓度梯度(10 mg/L,20 mg/L,40 mg/L,80 mg/L)和4个浸染时间梯度(8 h,16 h,24 h,32 h),分析茜素红染料在耳石上的沉积情况,以及不同暂养天数的动态变化,以期为大麻哈鱼标志放流技术及效果评估提供基础参考。结果表明,茜素红在矢耳石和微耳石上都能形成良好标记,在可见光和黄绿激光下均检测到标记带。随着暂养时间的延长,可见光下标记逐渐减弱,至80 d基本消失,而在黄绿激光下标记区能长久保持,荧光强度无减弱迹象。对不同时间点的标记级数随浓度变化关系进行曲线拟合,结合安全评估,得出适宜胚胎期大麻哈鱼耳石群体标记的条件为：浸染质量浓度(w/v)为25.9~40 mg/L,浸染时间为15.6~24 h,并且在二次函数y=0.055x2–3.861x+86.9(x表示时间值, y表示浓度值)之下的曲边三角形区域选择浓度和时间点。标记后不同采样时间点的新生轮纹数与暂养天数线性相关性显著,矢耳石和微耳石相关方程的斜率分别为0.965和0.924,表明大麻哈鱼胚胎-仔稚鱼后期矢耳石与微耳石上的沉积轮为日轮。%To explore otolith deposition with alizarin red S (ARS) immersed with conditions of different concentrations (10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L) and the time gradient (8 h, 16 h, 24 h, 32 h), as well as its marking results’ dynamic changes of different relaying days, artificial breeding chum salmon (Oncorhynchus keta) at the latter period of embryos were researched as immersing objects.This study tried for the protection of chum salmon with the approach of supplement for flag discharges, assessment for the releasing effects and provision for technical reference.The results showed that the sagittal and lapillus otoliths can be marked well, they all had great marked results detected with visible light and green laser light.The visible light marks were gradually weakened with the extension of sampling time, and were almost disappeared at the 80 d. And they were preserved with a long fluorescence intensity under the green laser without any sign of abating. Fitting curves were tried to express the relationship between the marking value and concentration for each series of time points. Combined with security assessments, the suitable group marking conditions for chum salmon otoliths at the stage of embryos were drawed, it was that lying the eggs with ARS immersed at the concentration of 25.9 mg/L to 40 mg/L , the time of 15.6 h to 24 h, and they must satisfy conditions that coordinates under the curve of quadratic function y=0.055x2–3.861x+86.9 (x means time, y means concentration). The new increased number of grain rounds were significantly correlated with cultured days in linear shape, and correlation equations of sagittal and lapillus otoliths were 0.965 and 0.924, which indicated that from embryos to late larvae, chum salmon otoliths deposited daily both in sagittal and lapillus.
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