目的：利用高压液相色谱法测定东北林蛙输卵管(林蛙油)、中华绒螯蟹卵(蟹黄)以及鲫鱼卵(籽)中雌二醇的含量。方法色谱柱为Agilent Zorbax SB-C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(63：37),检测波长280 nm,流速1.0 mL/min,柱温30℃,进样量为10μL。结果在0.3∼10μg/mL浓度范围内,高压液相色谱图峰面积与雌二醇浓度线性关系良好,其回归方程为Y=4203.8X-661.95,相关系数R=0.9991。林蛙油、中华绒螯蟹卵和鲫鱼卵中雌二醇的含量分别为3.00、0.42、0.54μg/g。结论超声波法可以有效提取样品中的雌二醇,通过优化所建立的方法对其具有良好的线性关系和回收率,高压液相色谱峰型美观、分离效果好。%Objective To determine the concentration of estradiol in Rana oviduct, ovum of Eriocheir si-nensis and ovum of Crucian carp by high pressure liquid chromatography. Methods The chromatographic column Agilent Zorbax SB-C18 (4.6 mm×250 mm, 5 μm) was used. Chromatographic conditions were as fol-lows:the mobile phase was acetonitrile-water (63:37), the detection wavelength was 280 nm, the flow rate was 1.0 mL/min, column temperature was 30 ℃, and sample amount was 10μL. Results A good linearity were obtained in the range of estradiol concentration 0.3∼10μg/mL with the above condition. Its regression equation was Y=4203.8X-661.95 with the correlation coefficient R=0.9991. The estradiol concentration of Rana oviduct, ovum of Eriocheir sinensis and ovum of Crucian carp were 3.00, 0.42, 0.54 μg/g, respectively. Conclusion The estradiol in these samples could be extract effectively by ultrasonic method. The optimized method has a good relationship and recovery rate for estradiol, and its peak pattern and separating effect of HPLC was good.
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