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首页> 中文期刊> 《法医学杂志》 >致死性脑干脑炎婴幼儿炎症细胞分布及PSGL-1的表达

致死性脑干脑炎婴幼儿炎症细胞分布及PSGL-1的表达

         
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目的：观察手足口病相关脑干脑炎致死的婴幼儿脑干中，炎症细胞分布特征及P选择素糖蛋白配体-1（P-selectin glycoprotein ligand-1，PSGL-1）阳性细胞的分布特点。方法收集20例手足口病相关的脑干脑炎死亡婴幼儿脑干组织蜡块作为实验组，10例因非脑部疾病和损伤死亡之婴幼儿脑干组织蜡块为对照组。应用免疫组织化学染色方法，检测多种类型炎症细胞免疫表型标志物和PSGL-1蛋白表达，观察阳性细胞分布特点。结果实验组脑干组织炎症细胞“袖套样”浸润的血管周围和胶质小结中，小胶质细胞分布最多，其次是中性粒细胞。小胶质细胞、中性粒细胞和淋巴细胞浸润程度之间差异具有统计学意义（P<0.05）。对照组脑干组织未见炎症细胞“袖套样”浸润的血管和胶质小结。实验组脑干组织PSGL-1广泛表达于炎症细胞，“袖套样”血管周围及胶质小结中阳性细胞数量多并且着色明显。两组的PSGL-1表达量差异具有统计学意义（P<0.05）。结论手足口病相关脑干脑炎死亡的婴幼儿脑干中，参与炎性发病过程的主要是小胶质细胞。 PSGL-1可能参与手足口病致死性脑干脑炎的疾病过程。%Objective To explore the distribution of inflam m atory cells and positive expression of P-se-lectin glycoprotein ligand-1 (PSG L-1) in infant brainstem tissue from hand-foot-m outh disease related fatal brainstem encephalitis. Methods Tw enty brainstem sam ples from infants suffered from brainstem en-cephalitis w ere collected as the experim ental group. Ten brainstem sam ples from infants died of non-brain diseases and injuries w ere collected as the control group. The distribution of inflam m atory cells and the expression of PSG L-1 in the tw o groups w ere exam ined by im m unohistochem ical m ethod. The characteristics of the positive cells w ere observed. Results In brainstem tissue of the experim ental group, there w ere sleeve infiltrations of inflam m atory cells around the vessels and in the glial nodule. Microglia was the m ost and following was neutrophils around the vessels and in the glial nodule. There was a significant statistical difference am ong m icroglias, neutrophils and lym phocytes (P<0.05). There was no sleeve infiltration in the control group. PSG L-1 protein was expressed w idely in inflam m atory cells in the experim ental group, especially in the inflam m atory cells around the vessels and in the glial nodule. B ut PSG L-1 positive staining could be observed significantly less in the control group com paring with the experim ental group (P<0.05). Conclusion Microglia is the m ain type of inflam m atory cells involved in the progress of the fatal disease. Moreover, PSG L-1 could participate in the pathogenesis of hand-foot-m outh disease related fatal brainstem encephalitis.

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