目的 研究小鼠脑内二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoicacid,DHA)含量对小鼠攻击行为的影响.方法 将雄性昆明小鼠72只随机分为对照组、鱼油组、辛伐他汀组和攻击参照组,每组18只.前3组严格隔离饲养,分别灌服生理盐水、深海鱼油和辛伐他汀3个月.在干预前及干预后对小鼠的攻击潜伏期、摇尾次数、攻击次数、攻击时间进行观测.在干预结束后利用气相色谱-质谱联用法定量检测小鼠脑组织中EPA和DHA的含量.攻击参照组仅作攻击性评估.结果 (1)干预前,各组小鼠的4项指标组间差异没有统计学意义,而干预后,各观测指标组间差异具有统计学意义(P＜0.05).(2)干预后,EPA和DHA的含量在鱼油组小鼠脑中均最高,在辛伐他汀组均最低.(3)小鼠EPA的含量与干预前、后4项指标的改变均呈负相关(P＜0.05),DHA含量与干预前、后摇尾次数和攻击次数的改变呈负相关(P＜0.05).结论 EPA和DHA含量与小鼠由应激所产生的攻击行为具有一定的因果关系.%Objective To explore the correlation between the eicosapentaenoic acid(EPA), docosahexaenoic acid (DHA) and the aggressive behavior in mice. Methods Seventy-two male Kunming mice were divided into control group, fish oil group, simvastatin group and aggressive reference group randomly. The control group, fish oil group and simvastatin group were given normal saline, fish oil and simvastatin by irrigation respectively for 3 months consecutively, each mouse was raised isolatedly. The latent period of assault, the frequencies of tail swing and assault, and the cumulative time of assault were recorded at the beginningand the end of the intervention. Finally, the EPA and DHA in brain were analyzed by gas chromatographymass spectrometry(GC-MS). The aggressive reference group was raised without intervention and was evaluated as aggressive reference only. Results (1) Before intervention, the latent period of assault, the frequencies of tail swing, the frequencies of assault, and the cumulative time of assault were not significantly different from each other group. After intervention, the differences were significant(P＜0.05). (2) After the intervention, the content of EPA and DHA in mice brain was the most in the fish oil group, and the least in the simvastatin group. (3) The content of EPA was negatively related with the four indexes (P＜0.05) before and after the intervention. The content of DHA was negatively related with the frequencies of tail swing and assault (P＜0.05 ). Conclusion There is a correlation between the EPA, DHA and aggressive behavior in mice under stress.
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