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首页> 外文期刊>林业研究（英文版） >Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.)
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Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.)

机译：抗生素对火炬松（Pinus taeda L.）植物器官再生的影响

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Three antibiotics ampicillin, carbenicillin, and cefotaxime were evaluated for their effects on induction, growth, and differentiation of organogenic calli, as well as rooting of regenerated shoots of three loblolly pine (Pinus taeda L.) genotypes. Of the antibiotics administered, cefotaxime maximally increased the frequency of callus formation and growth rate of organogenic calli, carbenicillin maximally increased the frequency of shoot regeneration and the average number of adventitious shoots per piece of organogenic callus, ampicillin maximally decreased the rooting frequency of regenerated shoots and mean number of roots per regenerated shoot, in comparison with antibiotic-free media. Compared with the control, ampicillin minimally increased the frequency of callus formation, cefotaxime minimally increased the frequency of shoot regeneration, and carbenicillin minimally decreased the rooting frequency of regenerated shoots in three loblolly pine genotypes tested. All three antibiotics increased the frequencies of callus formation and shoot regeneration, and reduced the rooting frequency of regenerated shoots suggested that the establishment of an efficient Agrobacterium tumefaciens-mediated transformation protocol for stable integration of foreign genes into loblolly pine need to select a suitable antibiotic. This investigation could be useful for optimizing genetic transformation of conifers.
机译：评价了三种抗生素氨苄青霉素，羧苄青霉素和头孢噻肟对三种器官基因型愈伤组织的诱导，生长和分化以及器官再生愈伤组织以及再生枝生根的影响。在所施用的抗生素中，头孢噻肟最大程度地增加了愈伤组织形成的频率和器官发生性愈伤组织的生长速率，羧苄青霉素最大程度地增加了芽再生的频率，每片器官发生性愈伤组织中不定芽的平均数目，氨苄青霉素最大程度地减少了再生芽的生根频率。与不含抗生素的培养基相比，每个再生芽的平均根数。与对照相比，在测试的三种火炬松基因型中，氨苄西林最小程度地增加了愈伤组织形成的频率，头孢噻肟最小程度地增加了芽再生的频率，羧苄青霉素最小程度地降低了再生芽的生根频率。这三种抗生素均增加了愈伤组织形成和芽再生的频率，并降低了再生芽的生根频率，这表明建立有效的根癌农杆菌介导的转化方案以将外源基因稳定整合到火炬松中需要选择合适的抗生素。这项研究可能对优化针叶树的遗传转化有用。

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