以组培出瓶10个月的8个品种大花蕙兰植株为试材,观测在10℃/0℃(日温/夜温)低温胁迫、25℃/15℃(日温/夜温)适温恢复条件下寒害指数、初始荧光值(F0)、PSⅡ系统最大光化学量子产量(Fv/Fm)、相对电导率、丙二醛(MDA)含量、可溶性糖含量和可溶性蛋白含量的变化,并对其抗寒性进行评价.结果表明:(1)随着低温胁迫时间的延长,不同品种大花蕙兰的F0、Fv/Fm、MDA含量、相对电导率和可溶性蛋白含量显著上升,可溶性糖含量先上升后下降,且随着抗寒性品种的不同其变化幅度和速度有所不同;(2)Fv/Fm与F0、MDA含量、相对电导率、可溶性蛋白含量均呈极显著相关,与可溶性糖含量显著相关,尤其与寒害指数的相关系数达94.4%,可作为抗寒性筛选的可靠指标之一;(3)8个品种大花蕙兰抗寒性强弱为:'金玉满堂'>'天之骄子'>'马可'>'黄金岁月'>'红梅'>'红霞'>'翠玉'>'福娘'.%To screen Cymbidium hybridium cultivar which adapts to cold climate in northern China, 8 varieties of 10-month C. hy-bridium were subject to cold stress for 1, 3 and 5 d, with daytime temperature being 10℃ and night temperature being 0℃ ( writ-ten as 10/0 ℃) . After returning to growth cabinet temperature ( 25/15 ℃) for the same duration of cold treatment, respectively, cold injury index, minimal fluorescence ( F0 ) , maximal photochemistry efficiency ( Fv/Fm ) , electric conductivity ( EC) , malonal-dehyde ( MDA) , soluble sugar content and soluble protein content were monitored, and followed by correlation analysis for compre-hensive evaluation. Results showed that F0 , Fv/Fm , MDA, EC and soluble protein content all increased as low-temperature treat-ment prolonged. While soluble sugar level firstly rose and then dropped. Magnitudes and rates of change varied among varieties. Fur-thermore, Fv/Fm was significantly correlated with F0 , EC, MDA and soluble protein content, and moderately correlated with soluble sugar. Correlation coefficient between Fv/Fm and chilling injury index was-0.944, indicating that Fv/Fm could be used as a reliable indicator of cold resistance of C.hybridium. Hardiness of 8 types of C.hybridium from high to low were C. 'Golden Yellow', C.'Lihi Tabidachi', C.'Buddy Mark', C. Lovely moon 'Croscent', C. 'Honey Heart', C. Royal Red Princess 'Nobuko', C. Plair'Days Memory' , C. 'Fuku Musume' .
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