以3-羟基-苯甲酸为内标,利用甲醇提取拟南芥叶片水杨酸,然后用三氯乙酸和盐酸溶解,再用乙酸乙酯:环己烷(1:1)萃取浓缩,高效液相色谱法测定.结果表明流动相0.2 mol?L-1醋酸钠:甲醇(9:1)pH为6.0时,水杨酸独立出峰,分离效果最好.用水稻及水仙叶片验证该提取及测定方法,效果良好,说明该方法在植物中具有一定的通用性.%To accurately determine salicylic acid ( SA) level in plant, methanol was used to extract SA from Arabidopsis leaf, with 3-hydroxy benzoic acid being the internal standard. After acid hydrolysis, the hydrolysate was partitioned with ethyl acetate and hexane mixture (1 : 1), and followed by high performance liquid chromatography (HPLC) analysis under different ratios and pH of the mobile phase. Results showed that SA was thoroughly separated from the complex mixture when methanol accounted for 10% of sodi-um acetate and methanol mixture under pH 6.0. Furthermore, rice and narcissus leaf was used to verify this method, whose results confirmed that this method was generally applicable for SA detection in plants.
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