应用改进的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法从感染柑橘速衰病毒(CTV)强株系和弱株系的墨西哥酸橙病株中检测到一种相对分子质量约为13000的特异蛋白,而在健株中未能检测到,该蛋白电泳谱带没有差异.应用Western印迹技术进一步分析表明,这种蛋白不与CTV特异的多克隆抗体1052和3DF1单克隆抗体反应.这暗示着该蛋白是墨西哥酸橙在CTV侵染胁迫条件下编码产生的一种病程相关蛋白.研究结果也表明,应用SDS-PAGE方法检测这一特异蛋白,可作为诊断墨西哥酸橙植株感染CTV的一种有效的分子生物学辅助检测方法.%A specific protein with a relative molecular mass of approximately 13 000 was detected in the total protein extracts from Mexican lime plants infected with severe or mild isolates of Citrus tristeza virus (CTV) by a modified sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) procedure.However,this specific protein was not detected in healthy plants.There were no differences among these specific proteins from Mexican lime seedlings infected with mild or severe isolates of CTV.Further analysis of the specific protein with Western blot techniques and CTV specific antibodies showed that the specific protein did not react with CTV coat protein specific polyclonal or monoclonal antibodies,1052 and 3DF1.It is suggested that the specific protein is a pathogenesis-related (PR) protein encoded by the Mexican lime plants infected with CTV.The results also indicated that the detection of this PR protein with the SDS-PAGE procedure is a useful biochemical method for detection of CTV in Mexican lime plants when the specific antibodies of CTV are not available.
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