中华蜜蜂精液在液氮(-196 ℃)中贮存121 d,精液与含有8%二甲亚砜的三羟基氨基甲烷缓冲稀释液以1∶1.5混合,不用平衡,在广口保温瓶中距液氮面2 cm或5 cm处预冻,在35～39 ℃水浴中解冻,可产生较好的解冻后活力比率(超过60%).当室温高于20 ℃时(4月份),样本必须于13 ℃下平衡0.5 h左右,其解冻后活力比率可达30%.春季采集并冷冻的样本,其解冻后活力不如冬季采集并冷冻的.用在液氮中贮存了1～121 d的精液分别以4～5 μL的剂量给24只处女王人工授精,4只开产但首先产的不是受精卵.解冻后的精液能够于13 ℃下保存46 h.磺胺不能用于精子冷冻.%Honeybee (Apis cerana cerana) semen was stored for 121 days at -196 ℃ in liquid nitrogen.Samples consistng of semen and tris buffer diluent containing 8% dimethyl sulphoxide,at 2 or 5 cm over the liquid nitrogen surface in a broadmouth thermos for 5-10 min to prefreeze and at 35-39 ℃ water bath to thaw,could produce over 60% post-thaw motility rate without equilibration(the radio between semen and tris buffer diluent was 1∶1.5).When the room temperature went beyond 20 ℃ (in April),samples must be equilibrated at 13 ℃ for 0.5 h or so,the post-thaw motility rate of the freezing sample would be over 30%.The post-thaw motility of samples collected and frozen in spring were not so good as that in winter,twenty four virgin queens were inseminated instrumentally with 4-5 μL semen stored for 1-121 days in liquid nitrogen,four of these queens laid but didn,t lay fertilized eggs first.Frozen semen could be stored for 46 h at 13 ℃ after thawing.Sulphanilamide should not be used in semen freezing.
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