Objective To investigate the antitumor activity of HB on KB cell in vitro and the inhibitory effect of chemical-induced hamster oral cancer. Methods MTT assay was used to detect the antitumor activity of HB on KB cell in vitro and chemical induction hamster oral cancerous. Methods: MTT, and then IC50 was calculated at 24, 48, 72 hours after the assay. The DMBA-induced cheek pouch cancer model was established by hamster (age at 8 weeks) and HB was used to intervene. Results HB inhibited the proliferation of KB cell in vitro , and IC50 of 24,48,72 hours was (12. 74 + 0. 13)mg/L,(4. 56 + 0. 22)mg/L and (2. 88 + 0. Ll)mg/L, respectively. The growth inhibition of HB on KB cell showed time and dose-dependent. The dysplasia incidence induced by HB 54, 72 mg/kg on DMBA-induced hamster oral mucosa was 66. 6% and 53. 3%, respectively. Compared with the control group(86. 6%), it decreased 20% and 33. 3%, respectively. Conclusion HB has significant inhibitory activity on KB cell and chemical-induced hamster oral cancer.%目的 研究黄癸素对体外培养人口腔上皮样癌KB细胞及化学诱导仓鼠的口腔癌变抑制作用.方法 MTT法检测黄癸素对KB肿瘤细胞株的影响,计算24,48,72 h的IC50.采用8周龄仓鼠,建立二甲基苯丙蒽(DMBA)诱发的颊囊癌模型,用黄癸素进行干预.结果 黄癸素对KB细胞有抑制作用,24,48,72 h的IC50分别为(12.74±0.13),(4.56±0.22),(2.88±0.11)mg/L;对KB细胞生长呈浓度、时间依赖性抑制作用.DMBA诱导的仓鼠在36,54,72 mg/kg的黄癸素作用下,颊黏膜不典型增生与癌变的发生率有不同程度的降低,其中54,72 mg/kg剂量组的病变总发生率分别为66.6%和53.3%,比模型组(86.6%)分别降低了20.0%和33.3%(P<0.01).结论 黄癸素能显著的抑制KB细胞生长,对化学物诱导的仓鼠口腔癌变也有抑制作用.
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