In this paper, a novel biodegradable poly (L-lactide)-polyethylenimine copolymer was successfully prepared from low-molecular-weight polyethylenimine and poly (L-lactide) using isophorone diisocyanate (IPDI) as the coupling agent.W/O/W emulsion-solvent evaporation method was used to make PEA microspheres for co-delivery of doxorubicin(DOX) and curcumin(Cur).The obtained PEA/(DOX+Cur) microspheres were characterized by detection of particle size, morphology study, drug loading, encapsulation efficiency, release study, cytotoxicity, cellular uptake and so on.This study suggested that PEA microspheres delivery system may help in development of co-delivery drugs to tumours and other diseases.%以辛酸亚锡(Sn(Oct)2)为催化剂,1,4-丁二醇和丙交酯为原料,开环聚合生成了聚乳酸(PLA),然后用异氟尔酮二异氰酸酯(IPDI)偶联小分子量的聚乙烯亚胺(2 000 Da)与PLA,生成了可生物降解的嵌段共聚物聚乳酸-聚乙烯亚胺(PLA-PEI,PEA).采用水包油包水(W/O/W)乳化溶剂挥发法,制备了共传输阿霉素(DOX)与姜黄素(Cur)的PEA微球联合给药系统.并对PEA/(DOX+Cur)微球进行了表征和考察,包括粒度分布、形貌、载药量、包封率、体外释放和细胞摄取等.通过实验结果可知,PEA微球给药系统有望为肿瘤及其它重大疾病联合治疗提供有意义的思路.
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