目的：探讨慢性脑低灌注白质损伤后脑周细胞 Kir6.1、Kir6.2的表达情况。方法：采用蛋白免疫印迹（Western blot）方法检测慢性脑低灌注白质损伤模型大鼠脑周细胞 Kir6.1、Kir6.2在不同时间点（术后第7 d、14 d、30 d、60 d）的表达变化。结果：（1）模型组在不同时间点 Kir6.1蛋白表达均升高，于30 d 达到高峰，与假手术对照组相比差异有统计学意义（P ﹤0.001）；（2）模型组在不同时间点 Kir6.2蛋白表达与假手术对照组相比差异无统计学意义（P ﹥0.05）。结论：慢性脑低灌注白质损伤后脑周细胞 Kir6.1表达增强，这提示 Kir6.1/ K-ATP通道可能参与了慢性脑白质损伤的病理生理过程；Kir6.1、Kir6.2的表达在脑周细胞出现了明显的异质性。%Objective:To investigate the expression of Kir6. 1、Kir6. 2 in the afterbrain pericyte after white matter injury under chronic cerebral hypoperfusion. Methods:The Western Blot method was used to detect the alterations of the Kir 6. 1、Kir6. 2 proteins in different periods of time(in the postoperative 7th,14th,30th,60th days)in the rat brain peri-cytes after white matter injury under chronic cerebral hypoperfusion. Results:(1)The expression of the Kir6. 1 protein in the model group was increased at different time. It was significantly different from that of the sham operation control group(P ﹤ 0. 01),and reached the peak in the postoperative 30th day;(2)The expression of the Kir6. 2 protein in the model group is not significantly different from that of the sham operation control group at different points of time(P ﹥0. 05). Conclusion:The expression of Kir6. 1 is enhanced in the afterbrain pericyte after white matter injury under chro-nic cerebral hypoperfusion. Kir6. 1 / K-ATP channel is involved in the pathophysiological process of chronic cerebral white matter injury;and the expressions of Kir6. 1、Kir6. 2 appear obvious heterogeneity in the brain pericytes.
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