着眼于利用EMS诱变蓝花丹种子,尝试通过不同处理方式获得诱变材料进行了研究.对诱变处理后的蓝花丹种子成苗情况及相关园艺性状进行了观察和实验分析,结果表明:蓝花丹种子的半致死剂量为0.5%,最佳处理时间为6 h;形态特征的观察发现EMS处理后代中出现了植株矮化、叶片狭长、花色变浅、花径变小或变大、雄蕊数目异常、叶片钙质结晶变少、花被腺点增多等现象;经过进一步的生理指标测定显示形态发生变化的植株丙二醛含量和可溶性糖含量均有不同程度的变化,这可能与抗性变化有一定的关系.%This research focused on getting mutagenic materials through different EMS treatments.The results showed that:the half lethal dose of Plumbago auriculata was 0.5%, the optimum processing time was 6 hours;Through the observation of morphological features found that mutation characters appeared including dwarf, long and narrow leaf, flowers light colored , flowers size changed ,abnormal stamen number, less Calcium crystal on the leaf, more glandular dots in perianth etc.;Further physiological index tests showed that malondialdehyde(MDA) and soluble sugars impact on the growth of Plumbago auriculata in different degree, which probably means resistant of these plants changed in some degree.
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