Four species ofmudskippers, including Periophthalmus,Boleophthalmus and Scartelaos, were caught in the mangrove area of China's Leizhou Peninsula,and 23cox1 gene sequences of these mudskippers were cloned.By analyzing the variation characteristics of mitochondrial cox1 genesand phylogenetic relationship of 16 species from the Leizhou Peninsula,Chinaand GenBank inother seaside,the efficacy of82mitochondrial cox1 geneswas evaluated as DNA barcodes formudskippers. Theresearch showed that genetic diversityin 82 homologouscox1 genes of 599bp was evaluated with 44 haplotypes, 212nucleotide polymorphismloci(35.4%)and 4average number of nucleotide differences (2.01%).Combingthe genetic distances of taxa withthe phylogenetic relation by using the neighbor joining (NJ) and maximum likelihood (ML),this article illustratedmost of Genus formed a monophyletic group,such as Periophthalmodon embedded in Periophthalmus,Boleophthalmus and Scartelaos formed aparaphyletic group.Therelaxed molecular clock was calibrated byfossil recordsto estimate the divergencedate.These estimates suggested thatthose Genus,including Periophthalmus, Periophthalmodon and others,separated about 23.61–15.65 Ma agoin the late Oligocene to the Early Miocene.%于我国雷州半岛红树林海区捕获隶属于弹涂鱼属(Periophthalmus)、大弹涂鱼属(Boleophthalmus)与青弹涂鱼属(Scartelaos)的4种弹涂鱼,克隆4种弹涂鱼23个个体cox1基因序列,通过分析4种弹涂鱼与GenBank中12种弹涂鱼cox1基因序列的变异特征,探讨4种弹涂鱼与后者的种间亲缘关系.结果表明,16种弹涂鱼的82个体的cox1基因序列共检测到单倍型44个,核苷酸多态位点212个(占35.4%),种间平均核苷酸差异数为4(占2.01%);基于最大似然法(ML)和邻接法(NJ)构建的系统发育树显示,各弹涂鱼属多形成一独立分支,亲缘关系明显,齿弹涂鱼属(Periophthalmodon)镶嵌于弹涂鱼属内,大弹涂鱼属与青弹涂鱼属形成并列分支,亲缘关系较近.依化石记录标定的松散分子钟估算物种分歧时间,结果表明,各弹涂鱼属出现明显分化大约发生于渐新世末期至中新世早期(23.61~15.65 Ma).
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