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首页> 中文期刊> 《广西医科大学学报》 >细胞色素b5还原酶2过表达对鼻咽癌细胞凋亡、细胞周期和侵袭的影响

细胞色素b5还原酶2过表达对鼻咽癌细胞凋亡、细胞周期和侵袭的影响

         
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目的:探讨细胞色素b5还原酶2(CYB5R2)基因过表达对鼻咽癌细胞凋亡、细胞周期和侵袭转移能力的影响.方法:将鼻咽癌细胞株CNE2和HONE1分为CYB5R2过表达组(转染CYB5R2-pCMV-Tag3A)和空载对照组(转染pCMV-Tag3A).RT-PCR验证CYB5R2基因过表达的鼻咽癌细胞模型的建立;流式细胞术检测细胞凋亡率和细胞周期;采用比色法检测细胞内Caspase 8和Caspase 9的活性;Transwell细胞侵袭实验评价细胞的侵袭能力.结果:CYB5R2过表达组鼻咽癌细胞中CYB5R2的转录水平显著高于对应的空载对照组细胞,CYB5R2基因过表达的鼻咽癌细胞模型建立成功.CYB5R2过表达组的鼻咽癌细胞凋亡率、Caspase 8和Caspase 9的活性显著高于对应的空载对照组(均P＜0.05);与对应的空载对照组比较,CYB5R2过表达组的鼻咽癌细胞S期比例明显减少,G0/G1期比例明显增加,且CYB5R2过表达组鼻咽癌细胞发生侵袭的细胞数目明显减少(均P＜0.05).结论:CYB5 R2过表达具有促进鼻咽癌细胞凋亡、周期阻滞和抑制侵袭转移的作用,可能成为鼻咽癌候选的抑癌基因.%Objective:To explore the influences of CYB5R2 gene on cell apoptosis,cell cycle and invasion and metastasis ability of nasopharyngeal carcinoma (NPC) cells.Methods:CYB5R2-pCMV-Tag3A and corresponding empty vector were transfected into CNE2 and HONE1 cells.The expression of CYB5R2 was confirmed by RT-PCR.Cell apoptosis and cycle were detected by flow cytometry.The activity of Caspase 8 and Caspase 9 were detected by colorimetric assay.Trans well invasive assay was used to evaluate the invasiveness of NPC cells.Results:The activity of Caspase 8,Caspase 9 and apoptosis rate in CYB5R2 overexpressing cells were significantly higher than those in control groups (P＜0.05).Compared with control,the proportion of S phase deduced while the proportion of G0/G1 phase was significantly increased in the CYB5R2 overexpressing cells.The number of invading cells in CYB5R2 overexpression group was significantly decreased as well (P＜0.05).Conclusion:Overexpression of CYB5R2 promotes cell apoptosis,arrests cell cycle and suppresses invasiveness of NPC cells,suggesting that CYB5R2 could be a potential antioncogene in treating NPC.

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