Objective: To explore the significance of vascular endothelial growth factors A (VEGF-A) and vascular endothelial growth factor receptor-2 (VEGFR-2) on angiogenesis in patients with cervical cancer. Methods: Serum VEGF-A and VEGFR-2 levels in 10 normal cervical tissues, 36 cases with cervical intraep-ithelial neoplasia CINⅢ, 43 cases with cervical carcinoma were detected by ELISA. Results: Serum VEGF-A and VEGFR-2 levels in cervical cancer group without lymph node metastasis and with lymph node metastasis were significantly higher than those in normal cervical tissue group and CINⅢ group ( P <0. 05). The levels of VEGF-A and VEGFR-2 were closely related with tumor size, stage, lymph node metastasis, but not related with the classification and organization. VEGF-A was linearly correlated with VEGFR-2. Conclusion: High levels of VEGF-A and VEGFR-2 play an important role in process of cervical carcinoma. VEGF-A and VEGFR-2 are related with the tumor size and lymph node metastasis. VEGF-A can enhance the incidence of lymph node metastasis in cervical cancer through VEGFR-2 signaling pathway.%目的:探讨血管内皮生长因子A(VEGF-A)及其血管内皮生长因子受体2(VEGFR-2)在宫颈癌血清中的表达及其在肿瘤发生、发展和转移中的作用.方法:采用酶联免疫分析法(ELISA)法检测正常宫颈组10例,宫颈上皮内瘤变CINⅢ组36例,宫颈癌组43例(无淋巴转移33例,淋巴转移10例)血清中VEGF-A、VEGFR-2的表达水平.结果:宫颈癌无淋巴结转移组和有淋巴转移组血清VEGF-A、VEGFR-2的表达水平均高于正常宫颈组及CINⅢ组,差异有统计学意义(P<0.05).VEGF-A 在宫颈癌血清中的表达水平与肿瘤大小、临床分期、淋巴结转移密切相关(P<0.05),但与组织分级和组织学类型无关(P>0.05).VEGFR-2 在宫颈癌患者血清中的表达水平与肿瘤大小、临床分期、淋巴结转移均密切相关(P<0.05),与组织分级、组织学类型无关(P>0.05).VEGF-A 和VEGFR-2 在宫颈癌血清中的表达水平呈正相关(Rs=0.211,P=0.030).结论:高水平血清VEGF-A、VEGFR-2表达在宫颈癌发生过程中起着重要的作用.宫颈癌患者血清中VEGF-A、VEGFR-2的表达水平均与肿瘤大小、淋巴结转移相关,VEGF-A可能在宫颈癌中通过VEGFR-2信号通道促进肿瘤血管形成导致肿瘤生长,并促成淋巴结转移.
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