Objective:To investigate the effect of allicin combined with vincristine on proliferation and apop-tosis in bladder cancer cell line BIU-87. Methods: BIU-87 cells with different treatments were divided into allicin, vincristine, and combination groups, and a non-drug treatment group was the blank control group. Inhibitory effect of allicin and vincristine on bladder cancer cell line BIU-87 proliferation was assessed by CCK-8 assay. The mRNA and protein levels of CDK1 and CyclinBl in BIU-87 cells were detected by reverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry and Western-blot. Cell apoptosis was analyzed by flow cytometry (FCM). Results: Anti-proliferation rate of bladder cancer cell line BIU-87 by allicin and vincristine treated is evident, in a dose-dependent manner. The combination group evidently reduced the expression of CDK1 and CyclinBl mRNA and protein in the BIU-87 cells, as compared with the blank control group ( P <0. 05). Both allicin and vincristine can also induce apoptosis. The apoptosis rate of combination group [(54. 89 ± 0. 27)%] was statistical significance ( P <0. 05) as compared with the blank control group [(3. 81±0. 63)%], the allicin group [(19. 20 ± 0. 26)%], and the vincristine group [(25. 39 ± 0. 31)%]; respectively. Conclusion: Allicin can enhance vincristine on the proliferation inhibition in bladder cancer cell line BIU-87, both of which have the synergistic antitumor effects; the mechanism of apoptosis induced by allicin may be associated with the down-regulation of the expression of CDK1 and CyclinBl.%目的:探讨大蒜素联合长春新碱对膀胱癌BIU-87细胞增殖和凋亡的影响.方法:实验分为大蒜素组、长春新碱组、大蒜素联合长春新碱组(联合用药组),以未用药物处理的BIU-87细胞为空白对照组.利用CCK-8法分别评价大蒜素和长春新碱对BIU-87细胞体外生长的抑制作用,通过逆转录聚合酶链反应(RT-PCR)、免疫细胞化学法和Western blot法检测4组细胞中CDK1和CyclinB1的mRNA和蛋白表达水平的变化,流式细胞术检测细胞凋亡情况.结果:大蒜素和长春新碱对膀胱癌BIU-87细胞的生长均有明显的抑制作用,呈浓度依赖性;联合用药组能明显降低BIU-87细胞中CDK1和CyclinB1的mRNA和蛋白的表达,与空白对照组相比差异均有统计学意义(P<0.05);同时能诱导细胞凋亡,联合用药组细胞凋亡率为(54.89±0.27)%,与空白对照组(3.81±0.63)%、大蒜素组(19.20±0.26)%、长春新碱组(25.39±0.31)%比较差异有统计学意义(P<0.05).结论:大蒜素可增强长春新碱对膀胱癌BIU-87细胞增殖抑制作用,两者具有协同抗肿瘤作用;大蒜素诱导细胞凋亡的机制可能与下调CDK1和CyclinB1的表达有关.
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