Objective:To evaluate effect of adipose-derived stem cells (ADSCs) on function of DNA damage repair in C57 mice with thymomas induced by irradiation.Methods:A total of 32 cleaning degree C57BL/6 mice were divided into four groups: control group of 8 mice with non-irradiation; irradiation group of 8 mice with irradiation by X-ray deep therapeutic apparatus; irradiation+ADSCs group of 8 mice, ADSCs were collected at 48 hours after transfection of EGFP expression plasmid, thymoma model mice were injected with 0.5 mL ADSCs at concentration 2.0í106/mL via tail vein one day after last irradiation; non-irradiation+ADSCs group of 8 mice with the same ADSCs injection as irradiation+ADSCs group. Four group mice were sacrificed on the 7th day after last irradiation; Western blot was applied to detect the expression of cyclinD1, c-myc, p53, ATM andγ-H2AX in mice thymus gland tissue.Results:Compared with irradiation group mice, the expression of ATM,γ-H2AX, cyclin-D1 and c-myc was significantly lower and the expression of p53 was significantly higher in irradiation+ADSCs group mice thymus gland tissue. When detected by flow cytometric, the percentage of G1 cells in irradiation group mice was significantly lower than that of control group, and the percentage of G1 cells in irradiation+ADSCs group mice was higher than that of irradiation group mice.Conclusion: ADSCs can reduce the degree of DNA damage and down-regulate the expression of p53 and c-myc proteins to induce G1 arrest and repair damaged thymocytes DNA to inhibit tumor growth.
展开▼
