目的:探讨恶性胸腔积液中RCAS1、Claudin-18表达量与增殖和侵袭基因表达的相关性.方法:87例原发性非小细胞肺癌合并恶性胸腔积液患者作为恶性胸腔积液组,56例结核性胸水患者作为结核性胸水组.对比两者胸水中RCAS1、Claudin-18基因,增殖、侵袭相关基因的表达量,采用Pearson检验评估恶性胸水中RCAS1、Claudin-18表达量与增殖和侵袭基因表达的相关性.结果:恶性胸水组胸水中RCAS1、Claudin-18 mRNA的表达量均显著高于结核性胸水组.恶性胸水组胸水中增殖基因LRRC3B、TCF21 mRNA的表达量低于结核性胸水组,SIRT1、EZH2 mRNA的表达量高于结核性胸水组;侵袭基因 DDX17、Nectin4、Vav3、NGAL、Snail mRNA 的表达量高于结核性胸水组,EFEM P1、MCPH1 mRNA的表达量低于结核性胸水组.Pearson检验发现,恶性胸腔积液中RCAS1、Claudin-18表达量与增殖相关基因、侵袭相关基因表达量直接相关.结论:恶性胸腔积液中RCAS1、Claudin-18表达量异常增高,且具体表达量与肿瘤细胞增殖侵袭活性直接相关,可作为鉴别良恶性胸水的可靠指标.%Objective:To investigate the correlation of RCAS1 and Claudin-18 expression with proliferation and invasion gene expression in malignant pleural effusion.Methods:187 patients with primary non-small cell lung cancer complicated by malignant pleural effusion were selected as malignant pleural effusion group and 56 patients with tuberculous pleural effusion were selected as tuberculous pleural effusion group.The expression of RCAS1 and Claudin-18 gene as well as proliferation and invasion-related genes in the pleural effusion were compared between the two groups,and Pearson test was used to evaluate the correlation of RCAS1 and Claudin-18 expression with proliferation and invasion gene expression in malignant pleural effusion. Results:RCAS1 and Claudin-18 mRNA expression in pleural effusion of malignant pleural effusion group were greatly higher than those of tuberculous pleural effusion group.Proliferation genes LRRC3B and TCF21 mRNA expression in pleural effusion of malignant pleural effusion group were lower than those of tuberculous pleural effusion group whereas SIRT 1 and EZH2 mR-NA expression were higher than those of tuberculous pleural effusion group;invasion genes DDX17,Nectin4,Vav3,NGAL and Snail mRNA expression were higher than those of tuberculous pleural effusion group whereas EFEMP1 and MCPH1 mR-NA expression were lower than those of tuberculous pleural effusion group.The Pearson test showed that the RCAS1 and Clau-din-18 expression in malignant pleural effusion were directly correlated with the expression of proliferation-related genes and in-vasion-related genes.Conclusions:RCAS1 and Claudin-18 expression increase abnormally in malignant pleural effusion,the specific expression is directly correlated with tumor cell proliferation and invasion activity,and they can be used as the reliable indicators for the identification of benign or malignant pleural effusion.
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