为了研究发酵牛粪作为奶牛卧床垫料的微生物消长规律,本试验选择舍饲散栏泌乳牛舍,检测1个自然年度牛粪垫料不同深度的微生物(细菌和真菌)数量.每2月检测1次,每次连续7d.结果表明,不同月份牛粪垫料的表、中、深层细菌数量随填铺卧床时间的增长表现出上升趋势,刚填铺的牛粪垫料细菌数量为2.0×106～3.8×107 cfu/g,填铺7d时细菌数量达4.7×107～3.6×108 cfu/g,4、6、8和10月份牛粪垫料的细菌数量均表现为表层偏高、中层偏低的趋势;牛粪垫料中真菌数量没有表现出明显的消长规律,且填铺7d时真菌数量增长幅度较小,刚填铺时牛粪的真菌数量介于4.3×102～1.5×103 cfu/g,填铺7d时真菌数量为9.5×102～4.8×103 cfu/g.此外,牛粪垫料的细菌和真菌数量与环境温度间均不存在显著相关性(P＞0.05).该研究可为奶牛卧床消毒制度的完善提供可靠数据,以提高奶牛的生产性能和健康水平.%The study aimed to investigate the microbial growth and decline in cubicle bedding of dairy cow dung.The free-stall cowshed was chosen to research the microbial (bacteria and fungus) count in different depths of cubicle bedding for a natural year.The research was carried out every two months,with 7 days for each time.The results revealed that the bacteria count in the surface,middle and deep layer of bedding increased with time extension of padding cubicle bed in all detected months.The bacterial count ranged from 2.0 × 106 to 3.8 × 107 cfu/g when the bedding was just filled,and the range was from 4.7 × 107 to 3.6 × 108 cfu/g after 7 days.The bacterial count in April,June,August and October showed a tendency that the count in surface layer was high and the count of middle layer was low.For fungi count,no significant difference was found among different lays or different months.The range of fungi count in bedding was from 2.0× 106 to 3.8× 107 cfu/g when the fermented bedding was just added into cubicle bed,and the count reached between 9.5 × 102 and 4.8 × 10a cfu/g after 7 days.Besides,the study showed that there was no significant correlation between microbial count in the bedding and ambient temperature (P＞0.05).This study would provide reliable data for the improvement of disinfection system in the cubicle bed of dairy cow to improve the performance and health level of the dairy cow.
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