通过研究鸡注射卵黄抗体后血清中抗体衰减的规律,为临床利用卵黄抗体治疗疾病提供理论指导.选取母源抗体衰减至近消失的60 d龄海兰灰公鸡随机分成对照组和试验组.试验组每只肌注2 mL含有新城疫、禽流感H5亚型和H9亚型3种抗体成分的卵黄抗体,对照组注射等量生理盐水.在注射后不同时间检测血清中3种抗体滴度的变化.结果显示试验组新城疫抗体在1～8 d均极显著高于对照组(P＜0.01)；试验组禽流感H5亚型抗体滴度在1～11 d极显著高于对照组(P＜0.01),注射抗体组禽流感H9亚型抗体滴度在1～14 d极显著高于对照组(P＜0.01).注射卵黄抗体后1d血清抗体即显著升高,5～6 d内抗体效价维持较高水平,然后逐步衰减.试验表明肌注卵黄抗体能够极显著提高鸡血清抗体滴度,注射后5d内抗体维持较高水平,半衰期大约为5d,之后随时间推移抗体滴度逐渐降低.证明肌注卵黄抗体治疗疾病具有起效快作用可靠的优点,值得临床推广应用.%This study on the dynamic change of chicken antibody after inject IgY aims to provide a guidance for clinical use of yolk antibody. 50 highland gray cocks whose maternal antibody attenuated to almost disappear randomly assigned into 2 groups. The experiment group were injected with 2 mL IgY including ND-Ab, AVI-Ab H5 and AVI-Ab H9 while the control group with 2 mL Sodium Chloride. The chicken serum antibody titer was measured by hemagglutina-tion inhibition test at different time. Results: the ND-Ab titer was significantly higher than that of control group in 1 - 8 day(F<0. 01), AVI-Ab H5 titer was significantly higher than the control group in 1 - 11 day(P<0. 01) ,and AVI-Ab H9 titer was significantly higher than the control group in 1 - 14 day(P<0. 01). The serum antibody titers increased significantly just Id post injection while the high antibody titers continued 5 - 6 days and then reduced gradually. The half life period of IgY in the serum was about 5days. It can be concluded that injecting the egg yolk antibody can significantly improve chicken serum antibody titer.
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