首页> 中文期刊> 《河北农业大学学报》 >干旱胁迫下AM真菌对丹参生长和养分含量的影响

干旱胁迫下AM真菌对丹参生长和养分含量的影响

         

摘要

以非灭菌土(农田土∶沙=2∶1)为生长基质,采用盆栽试验,研究了在土壤相对含水量为75%、50%和25%条件下接种 AM真菌对丹参(Salvia miltiorrhiza Bge.)生长和养分含量的影响.结果表明:干旱胁迫显著抑制了菌根侵染率并降低了植株生物量.不同土壤含水量下,接种 AM 真菌提高了丹参根系菌根侵染率、增加植株生物量、叶片可溶性糖、叶绿素、地下部全P、地下部全N及地下部丹参酮含量,降低地上部全N含量.接种株叶片可溶性蛋白和脯氨酸含量在土壤含水量为25%和50%时显著降低.接种株地上部和地下部总黄酮含量仅在土壤含水量为25%时显著降低,在土壤含水量为50%和75%时显著增加.接种AM真菌和干旱胁迫对丹参叶片可溶性糖、可溶性蛋白、脯氨酸、地上部全N、地上部和地下部全P、丹参酮及总黄酮含量均有显著交互作用.在土壤含水量为50%时接种效果最佳.AM真菌可能通过促进宿主植物根系对土壤水分及矿质元素的吸收和改善植物体内生理代谢活动来增强丹参植株抗旱性,促进丹参生长.%Effects of AM fungi on growth and nutritional contents of Salvia miltiorrhiza Bge.under 25% ,50% and 75% soil relative water content in pot culture with unsterilized soil(soil:sand=2:1) as growth substrate were studied. The results showed that AM fungi had significant influences on the growth of Salvia miltiorrhiza Bge. under different water content in soil.The mycorrhizal colonization rate and biomass of host plants significantly decreased under drought stress. Under different soil water conditions, inoculation with AM fungi promoted the AM fungal colonization of Salvia miltiorrhiza Bge. Inoculating AM fungi significantly increased the biomass, soluble sugar,chlorophyll in leaves and total P, total N and tanshinone contents in roots, and significantly decreased total N contents in leaves. The soluble protein and proline contents in leaves significantly decreased under 25% and 50% soil relative water content. The total flavonoids contents in leaves and roots significantly decreased under 25%soil relative water content, while significantly increased under 50% and 75% soil relative water content by AM fungal inoculation. Drought stress and AM had significant interaction effect on the contents of soluble sugar, soluble protein, proline and total N in leaves, total P, tanshinone and total flavonoid in leaves and roots. The best inoculation effect occurs under 50% soil relative water content. The mechanism of AM fungi strengthening drought resistance of mycorrhizal plant could be that AM fungal hypha contributed to soil water and minerals uptake in mycorrhizal plant, and AM fungi improved physiological metabolic activity of mycorrhizal plant. AM fungi could promote the growth of Salvia miltiorrhiza Bge..

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