为提高玉米芯中木聚糖得率,采用响应面法对碱法提取玉米芯木聚糖条件进行优化.利用Design - Expert 8的Min Run ResⅣ多因子两水平设计,对影响木聚糖提取的因素进行效应评价,利用最峭攀登搜索法为响应面分析的中心组合设计确定中心区,利用响应面分析得到优化提取条件.结果表明,碱提温度、碱提时间、NaOH质量分数和料液比对木聚糖提取率有显著影响,其最佳条件为:NaOH质量分数16％,温度85℃,料液比1∶16,提取时间2.5h.利用响应面法成功对玉米芯木聚糖提取条件进行优化,提取率达到25.6％.%The conditions of alkali extraction for increasing xylan yield from corn cob were optimized by response surface methodology (RSM). Min Run Res IV design of Design-Expert 8 was used to evaluate the influence of different factors,and then the steepest ascent path was employed to determine the central region of the medium composition for the further central composite design (CCD). Four factors which had significant effects were screened out,including temperature, time,concentration of NaOH and the ratio of solid to liquid. The optimal composition of these factors were 16% of NaOH,85℃,liquid/material ratio of 1 : 16,and 2. 5 h by RSM. Under the above conditions, the yield of corncob xylan was up to 25. 6%.
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