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Co60-γ射线诱变宝巾(Bougainvillea)的生物学效应研究

         

摘要

研究宝巾品种辐射处理后VM1代的生物学效应,为宝巾的辐射育种提供理论依据.以宝巾(B.Glabra)和毛宝巾(B.Spectabilis)为材料,通过不同剂量水平的Co60-γ射线处理,研究辐射剂量与其存活率、变异率的相关性,观察记录不同剂量下诱变株的生长表现,总结主要的变异性状类型.结果表明:在一定剂量Co60-γ射线范围内,辐射剂量和宝巾品种存活率呈负相关,和变异率呈正相关;毛宝巾的剂量敏感区域在50~150 Gy,而宝巾的剂量敏感区域可能在150~200 Gy,毛宝巾的辐射敏感性要高于宝巾;建立了毛宝巾剂量与存活率的线性回归方程:y=-0.409 8x+85.176,R2 =0.989 8,并由此计算毛宝巾的半致死剂量(LD50)为86 Gy;VM1代出现的各类叶变异性状主要为叶卷曲、叶残缺、叶皱缩、黄化叶等.%This paper studied the biological effect in VM1 generation of Bougainvillea varieties treated by Co60-γ ray and provided the theoretical reference for Bougainvillea irradiation breeding. The correlations of the dose and survival was studied,as well as variation rates by different dosage levels of Co60-γ ray with B. glabra and B. spectabilis. The growth performance for the variants was observed and recorded,and then the variation character types were generalized. The results showed that there is a negative correlation between dose and survival, but a positive between dose and variation rates. The radio sensitivity for B. spectabilis is superior to B. glabra. The regression equations were built with dose and survival for B. spectabilis: y= -0. 409 8x + 85. 176,R2=0. 989 8,calculating the half a lethal dose:86 Gy. In VM1 the leaf variations mainly were characterized by curly leaf,leaf deformity and leaf shrinkage,yellowing leaf,etc.

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