首页> 中文期刊> 《河南农业科学》 >过氧化物酶用于山楂嫁接亲和性早期鉴定研究

过氧化物酶用于山楂嫁接亲和性早期鉴定研究

         

摘要

木本植物嫁接体生长多年才表现出不亲和,为实现猩红英国山楂不同嫁接组合砧穗亲和性的早期鉴定,研究了猩红英国山楂及其砧木过氧化物酶(POD)的同工酶谱带,并对嫁接后各组合砧木和接穗的POD活性进行了动态测定.结果表明,砧穗POD同工酶谱带相同值的大小可以反映嫁接亲和性的强弱.嫁接后,砧穗POD活性差异小且逐渐变小的猩红英国山楂/单子山楂组合亲和性强,砧穗POD活性差异大且逐渐增大的猩红英国山楂/辽宁山楂组合亲和性差.因而认为,砧穗的POD活性可以作为山楂嫁接亲和性的早期鉴定指标.%Experiments were conducted to analyze the peroxidase isoenzymes of the ornamental hawthorn cultivar (Crataegus laevigata 'Paul's Scarlet') with three kinds of hawthorn root-stocks (C. pinatifida,C. sanguined,C. monogyna) , and to measure the POD activity of scion and rootstock bark after grafting. The results were as follows: the same count value of POD isoenzyme of scion and rootstock was related to graft-compatibility,t-he former was bigger and the latter was stronger. As growth of the graft union, the difference value of POD activity between rootstock and scion became smaller from Clps/Cm of which the compatibility was stonger, and from Clps/Cs, the difference value became bigger, while the compatibility was weaker. So the POD activity at graft unions could be used as a method for early detection of graft-compatibility.

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