采用寡营养与长时间培养方法,从活性污泥与土壤样品中分离筛选到7株高效聚磷菌.以菌株的除磷率为考察指标,确定其中的XF -5为高效聚磷菌株.对其培养条件进行优化,并进行了生理生化特性的检测与系统发育分析.16S rRNA基因序列的分析结果显示,XF -5与鞘氨醇杆菌属的菌种同源性达99％,结合生长特点与生理生化特性,初步确定该菌株为鞘氨醇杆菌(Novosphingobium sp.).当合成废水中总磷质量浓度为10.0 mg/L时,将菌株XF -5在28℃、pH值为6.5的条件下培养24 h,除磷率可达80％以上.该菌株在污水除磷与水体富营养化的防治方面,有潜在的应用前景.%A oligotrophic medium culture conditions was adopted to isolate and screen high phosphate accumulating bacteria from activated sludge and soil samples. After testing the phosphate accumulating rates,the strain XF-5 was chosen as a high efficient strain. In this study,physiological and biochemical characteristics and phylogenetic analysis were conducted for identification of the high efficient phosphorus removal strain. 16S rRNA gene sequence analysis showed that XF-5 and other species of the genus Novosphingobium had a homology of 99%. Combined with the growth and physiological and biochemical characteristics,XF-5 was finally identified as a member of Novosphingobium sp. When cultured in synthetic wastewater with total phosphorus concentration of 10 mg/L at 28 ℃ ,pH value of 6. 5 for 24 h,the phosphorus accumulation rate of the strain XF-5 could reach more than 80%. So the strain could be used to remove phosphorus of sewage and then used to control eutrophication.
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