To observe the effect of aloe polysaccharide(AP)on immune organs and immune efficacy of Newcastle disease(ND) vaccine in yellow broiler(broiler) ,AP solution was injected subcutaneously in amount of 3.75 ,7.50 ,11.25 ,15.00,0 mg each time per bird,respectively,while ND vaccine was inoculated. Effects of AP of different concentrations and injecting times on immune organ indexes and HI antibody titer in broilers vaccinated ND vaccine were studied. The results showed that AP could promote the growth of immune organs,increase the mass and indexes of thymus,spleen and bursa when injected to 5-day-old together with 1 dose live and 1/2 dose inactived ND vaccine,and enhance ND HI antibody titers of broilers vaccinated ND,especially the treatments of 3.75 ,7.50 mg AP per bird,in which effects were the most significant(P <0.05)on 35-day-old. AP improved immune organsand indexes when injected twice at 5 ,20-day-old,respectively,together with live ND vaccine,and the thymus indexes were significantly higher than those of the controled (P <0. 05) except the treatment of 15.00 mg AP per bird and the bursa indexes of all treatments were higher than those of the controled. AP also promoted ND HI antibody titer when injected twice at 5,20-day-old,respectively,together with live ND vaccine,ND HI antibody titers were higher than the controled after 15 days of first injection (20-day-old) .except the treatment of 15.00 mg AP per bird,especially the treatments of 3.75 mg AP per bird,in which effect was the most significant(P <0. 05) ,and ND HI antibody titers were significantly higher than those of the controled(P <0. 05) after 15 days of second injection(35-day-old) except the treatment of 11.25 mg AP per bird. It was proved that certain amount of AP can improve immune organs,enhance the immune efficacy of ND vaccine,and AP can be used as an immunopoten-tiator totether with ND vaccine.%为了观察芦荟多糖( Aloe polysaccharide,AP)对黄羽肉鸡免疫器官发育和新城疫(Newcastle disease,ND)疫苗免疫效果的改善和增强作用,在接种ND疫苗的同时颈部皮下注射AP溶液(AP实际注射剂量分别为每次3.75,7.50,11.25,15.00,0 mg·只-1),就AP不同质量浓度及其注射次数对肉鸡免疫器官指数和ND HI抗体滴度的影响进行了研究.结果表明,在5日龄接种ND活疫苗和半剂量灭活疫苗时1次注射AP,在一定程度上可促进免疫器官的发育,增加胸腺、脾脏和法氏囊的质量及其指数,但差异不显著(P＞0.05).除个别处理外,AP处理可提高ND疫苗免疫肉鸡的HI抗体滴度,并以试验后期(35日龄)3.75 mg·只-1和7.50 mg·只-1Ap处理者效果最显著(P＜0.05).在5日龄和20日龄接种ND活疫苗时,2次注射AP对免疫器官的发育和免疫器官指数有一定的影响.除15.00 mg·只-1AP处理外,其余各处理胸腺指数显著高于对照(P＜0.05)；AP各处理及与对照之间脾脏指数差异不显著(P＞0.05)；各处理法氏囊指数均高于对照,但差异不显著(P＞0.05).不同质量浓度的AP溶液对ND HI抗体滴度均有一定的影响.在第1次注射后15 d(20日龄),除15.00 mg·只-1Ap处理外,其余各处理ND HI抗体滴度均高于对照,但只有3.75 mg·只-1AP处理者差异显著(P＜0.05)；而在第2次注射后15d(35日龄),除11.25 mg·只-1Ap处理外,其余各处理肉鸡ND HI抗体滴度均高于对照,且差异显著(P＜0.05).由此证明,一定质量浓度的AP可改善免疫器官的发育,提高ND疫苗免疫效果,并可以作为免疫促进剂与ND疫苗同时使用.
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