β-伴大豆球蛋白是7S球蛋白的主要成分,是重要的大豆抗原蛋白.在单因素试验的基础上对超高静压处理压强、处理时间、β-伴大豆球蛋白质量浓度3个因素进行研究,以β-伴大豆球蛋白抗原抑制率为响应值,采用响应面法优化β-伴大豆球蛋白超高静压处理条件.结果表明:β-伴大豆球蛋白超高静压处理最佳条件为压强455 MPa、时间18 min、蛋白质量浓度15 mg/mL,在此条件下β-伴大豆球蛋白抗原性抑制率为49.59％.验证试验测得此条件下β-伴大豆球蛋白抗原抑制率为51.19％,标准偏差为1.07％,说明利用响应面法分析结果可靠.%β-conglycinin is the major component of the globulin,and is an important soybean antigen protein.The present study was to investigate the optimal high hydrostatic pressure treatment parameters for reducing Antigenicity of β-conglycinin by response surface methodology.Based on the single-factor experiments,the effects of HHP treatment pressure,HHP treatment time and β-conglycinin concentration on the antigenicity of β-conglycinin were studied.The results showed that the optimal high hydrostatic pressure parameters process conditions were as follows:HHP treatment pressure of 455 MPa,HHP treatment time of 18 min and β-conglycinin concentration of 15 mg/mL.Under these conditions,the predicted and observed antigenicity inhibition rates of β-conglycinin were 49.59％ and 51.19％,respectively.The standard deviation was 1.07％,which implied that the optimal conditions of HHP treatment obtained by response surface methodology was reliable.
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