Objective To investigate the induction of apoptosis in human pancreatic cancer cell Panc-l by Solanine, Methods Solanine of various available concentrations (20, 30, 40, 50 μg/mL) were added to Panel cell which stayed in logarithmic phase for the purpose of inhibiting; the growth of Panc-l cells. The morphology of cells was observed by inverted microscope; the inhibitory effect was detected with CCK-8 colorimetric method; and the cells apoptosis rate was detected with flow cytometry in Annexin V~FITC/PI double staining method. Results Solanine effectively inhibited the growth of Pane-1 in a manner of concentration-time dependent. Pane-1 presented typical apoptotic morpho-logieal changes with the increase of drug concentration and response time under the observation of inverted microscope. And the inhibitory effect for the proliferation of Panel gradually enhanced in a time-concentration dependent manner. Meanwhile, the apoptosis rate gradually increased with the increasing of drug concentration. Conclusion Solanine inhibited the proliferation of pancreatic cancer ceils in a time and concentration dependent manner. Furthermore, the inhibitory effect may be achieved by the apoptosis induction effect of Solanine in a manner of concentration dependent.%目的 探讨龙葵碱对胰腺癌细胞Panc-1的凋亡诱导作用.方法 采用经典方法培养胰腺癌细胞系Panc-1成功后,取对数生长期的细胞用于实验研究,用不同浓度(分别为20、30、40和50 μg/mL)的龙葵碱进行干预,采用倒置显微镜观察龙葵碱作用后Panc-1细胞的形态学改变；CCK-8比色法检测龙葵碱对Panc-1细胞增殖的抑制作用；用流式细胞术Annexin V-FITC/PI双染法检测细胞凋亡率.结果 龙葵碱显著抑制人胰腺癌细胞Panc-1生长,并具有浓度和时间依赖性.倒置显微镜观察发现,随着龙葵碱浓度增加和作用时间延长,Panc-1细胞表现出典型的凋亡形态改变；CCK-8比色法发现,随着龙葵碱浓度增加和作用时间延长,对Panc-1细胞的增殖抑制作用逐渐增强；流式细胞术检测发现,Panc-1细胞的凋亡率随着龙葵碱浓度增加而逐渐增加.结论 ①龙葵碱能够抑制胰腺癌细胞Panc-1的增殖,且此抑制作用具有时间和浓度依赖性.②龙葵碱具有诱导胰腺癌细胞Panc-1凋亡的作用,并具有浓度依赖性.
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