首页>
中文期刊>
《当代医学科学(英文)》
>Preparation of Magnetic Molecularly Imprinted Polymers for Selective Isolation and Determination of Kaempferol and Protoapigenone in Macrothelypteris torresiana
Preparation of Magnetic Molecularly Imprinted Polymers for Selective Isolation and Determination of Kaempferol and Protoapigenone in Macrothelypteris torresiana
Novel uniform-sized magnetic molecularly imprinted polymers(MMIPs) were synthesized for selective recognition of active antitumor ingredients of kaempferol(KMF) and protoapigenone(PA) in Macrothelypteris torresiana(M. torresiana) by surface molecular imprinting technique in this study. Super paramagnetic core-shell nanoparticles(γ-MPS-SiO 2@Fe3O4) were used as seeds, KMF as template molecule, acrylamide(AM) as functional monomer, and N, N'-methylene bisacrylamide(BisA M) as cross-linker. The prepared MMIPs were characterized by X-ray diffraction(XRD), Fourier transform infrared spectrum(FTIR), transmission electron microscopy(TEM) and thermo-gravimetric analysis(TGA), respectively. The recognition capacity of MMIPs was 2.436 times of non-imprinted polymers. The adsorption results based on kinetics and isotherm analysis were in accordance with the pseudo-second-order model(R2=0.9980) and the Langmuir adsorption model(R2=0.9944). The value of E(6.742 k J/mol) calculated from the Dubinin-Radushkevich isotherm model suggested that the physical adsorption via hydrogen-bonding might be predominant. The Scatchard plot showed a single line(R2=0.9172) and demonstrated the homogeneous recognition sites on MMIPs for KMF. The magnetic solid phase extraction(MSPE) based on MMIPs as sorbent was established for fast and selective enrichment of KMF and its structural analogue PA from the crude extract of M. torresiana and then KMF and PA were detected by HPLC-UV. The established method showed good performance and satisfactory results for real sample analysis. It also showed the feasibility of MMIPs for selective recognition of active structural analogues from complex herbal extracts.
展开▼
机译:The preparation and the in-vitro pharmacodynamics study of the intracapsular sustained-release preparations for the prevention of posterior capsule opacification
机译:How the Group Leaders of Lesson Preparation Groups Facilitate Group Learning and Reflective Practice in Chinese Public High Schools =中国公立高中的备课组长是如何引领团队学习和反思性实践的?
机译:神父市政厅,宗教自由和天主教传统。真实地狱中的均一教义发展案例,阿巴耶·圣马德琳(Les Barroux),第二版。修订和扩充,1998年,T。I,系统学,第一期:Preparations,LX + 466页。第二期:成果,278页。 T. II,编年史,总理专着:资料来源,第594页。第二期:所附文件,430页。 T. III,按字母顺序,第一期:参考书目,552页。第二期:一般分析索引,540页。