Objective To observe the different concentration of pomegranate peel polyphenols ointment on histopathologic morphology in rabbit ear acne model, and to determine the best concentration of pomegranate peel polyphenols ointment on acne. Methods The 2% coal tar daub solution about 0.5 mL was smeared at about 2.0 cm x 2.0 cm range of rabbit left and right outer edge, once daily for two weeks to make rabbit ear acne model. The different concentration (18%, 9%, 3%) of pomegranate peel polyphenols ointment was prepared, which was smeared evenly on the rabbit ear acne model, twice daily for four weeks. The histopathologic changes were observed under light microscopy. Results The medium dose of pomegranate peel polyphenols group and the control group (isotretinoin) showed the most obvious effect on the pathological histology of acne model in the degree of inflammation, keratinization, hair follicles expansion. Compared with blank group, the degree of inflammation, keratinization, hair follicles expansion of model group was more significant (P <0.01). Compared with the model group, the medium dose of pomegranate peel polyphenols group and the control group could significantly decrease the degree of inflammation, keratinization, hair follicles expansion (P<0.01); the low dose and high dose of pomegranate peel polyphenols groups also could obviously decrease the degree of inflammation, keratinization, hair follicles expansion (P<0.05), the decreased degree in which was lower than medium dose of pomegranate peel polyphenols group and the control group (P<0.05). Conclusion The medium dose of pomegranate peel polyphenols group and the control group show the most significant anti-keratinization and anti-inflammatory effect on the rabbit ear acne model.%目的 观察不同浓度的石榴皮多酚软膏对兔耳粉刺模型组织病理形态学的影响,确定痤疮治疗的最佳石榴皮多酚软膏浓度.方法 采用家兔左、右外耳道外缘处约2.0 cm×2.0 cm范围涂抹2%煤焦油溶液约0.5 mL,1次/d,连续2周以制成动物粉刺模型.将不同浓度(18%、9%、3%)的石榴皮多酚制备成软膏,均匀涂抹于兔耳粉刺模型,2次/d,连续4周.取材后在光镜下观察兔耳粉刺模型组织病理形态的变化.结果 石榴皮多酚中剂量组与阳性药物(异维A酸)组对粉刺模型病理组织形态学兔耳炎症程度、角化程度、毛囊扩张程度的影响最为显著;模型组与空白组相比,兔耳炎症、角化、毛囊扩张显著(P<0.01);与模型组相比,石榴皮多酚中剂量组与阳性药物组均能显著降低兔耳炎症程度、角化程度、毛囊扩张程度(P<0.01);石榴皮多酚高剂量组与石榴皮多酚低剂量组也能降低兔耳兔耳炎症程度、角化程度、毛囊扩张程度(P<0.05),但不如石榴皮多酚中剂量组与阳性药物组(P<0.05).结论 石榴皮多酚中剂量组及阳性药物组对兔耳粉刺模型的抗角化及抗炎作用最显著.
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