Objective To discuss the influence of blood-activating(angelica sinensis and ligusticum wallichii) and blood-breaking medicines(rhizoma sparganii and curcuma zedoary) on the PCNA protein and VEGFmRNA,VEGFR-2 mRNA expressions in the aortic tunica intima of rats with atherosclerosis (AS).Methods 60 male Wistar rats were randomly divided into 5 groups (including blank group,model group,statins group,blood-activating medicine group and blood-breaking medicine group) and AS rat model was established by feeding high-fat forage.These medicines were given by intragastric administration for 4 weeks.After treatment,PCNA protein expression level was detected with immunohistochemical staining method and the VEGF mRNA,VEGFR-2 mRNA gene expression levels were tested with hybridization in situ of the aortic tunica intima.Results The positive cell amount of PCNA in the blood-breaking medicine group was lower than that in the blood-activating medicine group (P＜0.05).The VEGF mRNA and VEGFR-2 mRNA average gray values in the model group were higher than those in the blank group,statins group,blood-activating medicine group and blood-breaking medicine group,while there were no significant differences between blood-activating medicine group and blood-breaking medicine group.Conclusion Angelica sinensis,Ligusticum wallichii,rhizoma sparganii and curcuma zedoary can inhibit the expression of PCNA protein,VEGF mRNA and VEGFR-2 mRNA,and then inhibit the cell proliferation.The inhibiting effects on the PCNA expression of blood-breaking medicines (rhizoma sparganii and curcuma zedoary) are better than those of bloodactivating medicines(Angelica sinensis and Ligusticum wallichii).%目的 探讨活血药(当归、川芎)、破血药(三棱、莪术)对动脉粥样硬化(AS)的大鼠主动脉增殖细胞核抗原(PCNA)蛋白及血管内皮生长因子(VEGFmRNA)、血管内皮生长因子受体(VEGFR-AS)表达的影响.方法 将60只雄性Wistar大鼠随机分为5组(空白组、模型组、他汀组、活血组、破血组),先采用高脂喂饲AS大鼠,造模成功后,灌胃给药,连续4周.实验结束后,用免疫组化染色法检测主动脉内膜PCNA蛋白表达；用原位杂交法检测主动脉内膜VEGFmRNA、VEGFR-2mRNA的基因表达.结果 破血组PCNA阳性细胞数低于活血组(P＜0.05)；模型组VEGFmRNA、VEGFR-2mRNA平均灰度值均高于空白组、他汀组、活血组和破血组(P＜0.05)；活血组、破血组组间比较无差异.结论 当归、川芎与三棱、莪术均能抑制PCNA、VEGFmRNA及VEGFR-2mRNA的表达,从而起到抑制细胞增殖的作用.在抑制PCNA表达方面,破血药三棱、莪术优于活血药当归、川芎.
展开▼
