A rapid analytical method was developed for the determination of ibuprofen arginine by contactless conductivity detection with microfluidic chip.Influence parameters on separation and detection such as type and concentration of buffer,additive,separation voltage and injection time were investigated and optimized.Under the optimum conditions,i.e.20 mmol/L Tris-20 mmol/L H3 BO3 (pH 8.6) as buffer solution,no additive,a separation voltage of 2.0 kV and an injection time of 10.0 s,ibuprofen arginine could be separated and detected within 45.0 s.The results showed that good linearities for ibuprofen and arginine existed in 80.0-1.00 × 103 mg/L,with their correlation coefficients(r) of 0.998 and O.997,respectively.The limits of detection (S/N=3) for them were 60 mg/L with RSDs of 1.9％ and 1.8％,respectively.And their recoveries were in the ranges of 97.9％ -103％ and 97.3％-102％,respectively.This method was rapid and simple,and could be used for the detection and quality control of non-steroidal arginine ibuprofen anti-inflammatory drugs.%建立了微流控芯片非接触电导检测快速测定精氨酸布洛芬含量的方法.考察了缓冲液种类和浓度、添加剂、分离电压以及进样时间等因素对分离检测的影响.优化条件为:20 mmol/L Tris-20 mmol/L H3 BO3(pH 8.6)为缓冲溶液、不加添加剂、分离电压2.0 kV、进样时间l0.0s,在45.0 s内可实现精氨酸布洛芬的快速分离测定.结果表明,布洛芬和精氨酸在80.0 ～ 1.00×103 mg/L范围内线性关系良好,相关系数(r)分别为0.998和0.997,检出限(S/N=3)为60 mg/L,相对标准偏差分别为1.9％和1.8％,加标回收率分别为97.9％～ 103％和97.3％ ～102％.该方法快速、简便,为精氨酸布洛芬非甾体抗炎药物的分析和质量控制提供了一种新方法.
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