采用胶束毛细管电泳（ MEKC），建立了四环素（ TCs）和青霉素（ PENs）两类7种药物同时分离的方法。考察了MEKC中缓冲液类型、离子浓度和pH值，以及表面活性剂（ SDS）浓度、分离电压、温度等参数的影响。利用L16（45）正交试验，确立了最佳的电泳条件：缓冲液为40 mmol/L 磷酸二氢钾-20 mmol/L 硼砂，添加65 mmol/L SDS，pH 7.9，分离电压28 kV，分离温度28℃，紫外检测波长分别为350 nm 和200 nm。结果表明：7种药物在25 min内得到完全分离。在1.56~50 mg/L 范围内呈良好的线性关系，相关系数（r2）为0.9979~0.9999，峰面积的相对标准偏差（n=6）为4.1％~7.3％；迁移时间的相对标准偏差（n=6）为0.33％~0.67％。在2.0，5.0，10.0 mg/kg的加标水平下，7种药物的回收率为83.6％~93.3％，相对标准偏差（ n=6）为4.7％~7.6％。该法快速、简便、准确，具有较高的灵敏度，已应用于合肥市及周边地区水塘和湖水中7种药物的快速分离检测。%A method for the simultaneous separation of seven kinds of drugs including tetracyclines (TCs)and penicillins(PENs)by micelle capillary electrophoresis(MEKC)was developed. Effects of some important factors such as composition,pH value and concentration of running buffer,surfac-tant concentration,separation voltage and separation temperature were investigated,and the factors were optimized by the L16(45 )orthogonal test. The optimal conditions were as follows:the running buffer:40 mmol/L potassium dihydrogen phosphate -20 mmol/L borax solution( pH 7. 9 ), SDS concentration:65 mmol/L,separation voltage:28 kV,separation temperature:28 ℃,UV detec-tion wavelength:350 nm and 200 nm. Seven kinds of drugs were baseline separated within 25 min. The calibration curves showed good linearities in the range of 1. 56-50 mg/L,with correlation coef-ficients(r2)of 0. 997 9-0. 999 9. RSDs(n=6)were in the range of 4. 1% -7. 3% for peak area and 0. 33% -0. 67% for migration time. The average recoveries of seven kinds drugs at three spiked concentration levels of 2. 0,5. 0,10. 0 mg/kg ranged from 83. 6% to 93. 3% ,with RSDs(n=6)of 4. 7% -7. 6% . The method was rapid,simple,accurate and high sensitive,and was successfully applied in the determination of seven kinds of drugs in the ponds and lakes of Hefei and its surround-ing areas.
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