采用喷射式电化学发光流动检测技术建立了奶粉和液态奶中三聚氰胺检测的新方法.在优化条件下,三聚氰胺浓度的负对数在1.0 ×10-9～1.0 ×10-3 g/L范围内与其电化学发光强度呈良好的线性关系,检出限(S/N=3)为1.0×10-9 g/L.不同加标量的阴性样品加标回收率为92.2％～114.2％,相对标准偏差为0.469％～ 1.2％.该方法具有检测灵敏、操作简单、样品用量少等优点,有望用于三聚氰胺的现场快速检测.%Based on the improved jet type electrochemiluminescence (ECL) flow injection system,a sensitive method for the determination of melamine in milk power and milk was developed.The experimental conditions of the proposed method,for example pH value,flow rate,injection volume,scan rate and concentration of Ru (bpy)32+ were optimized.The best results were achieved under the following conditions:running buffer:0.1 mol/L pH 9.5 phosphate buffer,detection reagent:2.5 × 10-4 mol/L Ru(bpy)3+2,flow rate:20 μL/s,injection volume:100 μL,scan rate:50 mV/s.Under the optimized conditions,the ECL intensity was proporational to the negative logarithmic concentration of melamine in the range of 1.0 × 10-9-1.0 × 10-3 g/L.The detection limit (S/N =3)was 1.0 × 10-9 g/L.The recoveries of melamine in milk power and milk at various spiked levels were in the range of 92.2％-114.2％ with relative standard deviations (RSDs) of 0.46％-1.2％.The proposed method,with the advantages of sensitivity,easy to operation,and low sample consumption,is expected to be used for the rapid detection of melamine on spot.
展开▼
