用实时荧光定量PCR方法检测56对人卵巢癌组织及对应的癌旁组织中iASPP( Inhibitor of ASPP family) mRNA表达水平,应用受试者工作特征曲线(Receiver operating characteristic curve,ROC曲线)分析癌组织与癌旁组织中iASPP mRNA表达水平,探索iASPP在卵巢癌发生中的作用.从细胞水平进一步研究其作用机制,用siRNA干扰的方法使iASPP表达降低后用Hoechst 33342和CCK-8分别检测iASPP沉默后对人类卵巢癌细胞株OVCA420的影响.结果显示,卵巢癌组织中iASPP表达较癌旁组织明显增高；iASPP沉默后,细胞凋亡增多,细胞增殖水平降低.据此推断,iASPP mRNA水平对卵巢癌的临床诊断具有一定价值,可作为卵巢癌治疗的一个重要靶点.%To investigate the role of iASPP in human ovarian cancer progression, the expression levels of iASPP in 56 pairs of ovarian cancer tissues and the matched tissues were examined using quantitative real-time PCR. Receiver operating characteristic(ROC) analysis was performed to further evaluate the clinical value of iASPP mRNA expression for the prediction of ovarian cancer. The iASPP gene expression could be firstly silenced by an iASPP - siRNA interference method in vitro. Then, the biological impact was investigated by Hoechst 33342 and CCK-8 assay in ovarian cell line OVCA420. Comparing with the adjacent tissues, it showed an over-expressed iASPP in human ovarian cancer tissues. Down-regulation of siRNA mediated by iASPP resulted in inhibited proliferation and increased apoptosis of ovarian cancer cell line OVCA420. According to the results, iASPP, which showed over-expression in the progression of ovarian cancer, could be considered as a target for ovarian cancer therapy. Moreover, iASPP mRNA could be a candidate marker for the diagnosis of o-varian cancer.
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